Integrated recovery of pigments released from red beet hairy roots exposed to acidic medium

Usha Mukundan, Veena Bhagwat, Gurmeet Singh, Wayne Curtis

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Hairy root cultures of Beta vulgaris L grown in a bubble column reactor were permeabilised by exposure to B5 medium of pH 2.0. The roots released 39% of their total pigments on a 10 min exposure to B5 medium of pH 2.0 followed by return to standard B5 medium. The pigments released in the extracellular medium were recovered on an adsorption column containing XAD-16 resin. The permeabilised roots regrew and accumulated additional pigments. Comparison of this technique with the previously used techniques like oxygen starvation and temperature shock to permeabilise beet hairy roots suggest that pH mediated release of betalains can be an effective method of releasing betalains from root cultures.

Original languageEnglish (US)
Pages (from-to)67-69
Number of pages3
JournalJournal of Plant Biochemistry and Biotechnology
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1 2001

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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