Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity

Daniel H. Janzen, Winnie Hallwachs, Patrick Blandin, John M. Burns, Jean Marie Cadiou, Isidro Chacon, Tanya Dapkey, Andrew Robert Deans, Marc E. Epstein, Bernardo Espinoza, John G. Franclemont, William A. Haber, Mehrdad Hajibabaei, Jason P.W. Hall, Paul D.N. Hebert, Ian D. Gauld, Donald J. Harvey, Axel Hausmann, Ian J. Kitching, Don LafontaineJean Fran Çois Landry, Claude Lemaire, Jacqueline Y. Miller, James S. Miller, Lee Miller, Scott E. Miller, Jose Montero, Eugene Munroe, Suzanne Rab Green, Sujeevan Ratnasingham, John E. Rawlins, Robert K. Robbins, Josephine J. Rodriguez, Rodolphe Rougerie, Michael J. Sharkey, M. Alex Smith, M. Alma Solis, J. Bolling Sullivan, Paul Thiaucourt, David B. Wahl, Susan J. Weller, James B. Whitfield, Keith R. Willmott, D. Monty Wood, Norman E. Woodley, John J. Wilson

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

Inventory of the caterpillars, their food plants and parasitoids began in 1978 for today's Area de Conservacion Guanacaste (ACG), in northwestern Costa Rica. This complex mosaic of 120 000 ha of conserved and regenerating dry, cloud and rain forest over 0-2000 m elevation contains at least 10 000 species of non-leaf-mining caterpillars used by more than 5000 species of parasitoids. Several hundred thousand specimens of ACG-reared adult Lepidoptera and parasitoids have been intensively and extensively studied morphologically by many taxonomists, including most of the co-authors. DNA barcoding - the use of a standardized short mitochondrial DNA sequence to identify specimens and flush out undisclosed species - was added to the taxonomic identification process in 2003. Barcoding has been found to be extremely accurate during the identification of about 100 000 specimens of about 3500 morphologically defined species of adult moths, butterflies, tachinid flies, and parasitoid wasps. Less than 1% of the species have such similar barcodes that a molecularly based taxonomic identification is impossible. No specimen with a full barcode was misidentified when its barcode was compared with the barcode library. Also as expected from early trials, barcoding a series from all morphologically defined species, and correlating the morphological, ecological and barcode traits, has revealed many hundreds of overlooked presumptive species. Many but not all of these cryptic species can now be distinguished by subtle morphological and/or ecological traits previously ascribed to 'variation' or thought to be insignificant for species-level recognition. Adding DNA barcoding to the inventory has substantially improved the quality and depth of the inventory, and greatly multiplied the number of situations requiring further taxonomic work for resolution.

Original languageEnglish (US)
Pages (from-to)1-26
Number of pages26
JournalMolecular Ecology Resources
Volume9
Issue numberSUPPL. 1
DOIs
StatePublished - Jan 1 2009

Fingerprint

barcoding
Biodiversity
DNA barcoding
biodiversity
DNA
Equipment and Supplies
Costa Rica
Wasps
Butterflies
Edible Plants
Lepidoptera
Moths
parasitoids
Mitochondrial DNA
Diptera
Libraries
insect larvae
caterpillar
Tachinidae
tropical montane cloud forests

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Janzen, D. H., Hallwachs, W., Blandin, P., Burns, J. M., Cadiou, J. M., Chacon, I., ... Wilson, J. J. (2009). Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity. Molecular Ecology Resources, 9(SUPPL. 1), 1-26. https://doi.org/10.1111/j.1755-0998.2009.02628.x
Janzen, Daniel H. ; Hallwachs, Winnie ; Blandin, Patrick ; Burns, John M. ; Cadiou, Jean Marie ; Chacon, Isidro ; Dapkey, Tanya ; Deans, Andrew Robert ; Epstein, Marc E. ; Espinoza, Bernardo ; Franclemont, John G. ; Haber, William A. ; Hajibabaei, Mehrdad ; Hall, Jason P.W. ; Hebert, Paul D.N. ; Gauld, Ian D. ; Harvey, Donald J. ; Hausmann, Axel ; Kitching, Ian J. ; Lafontaine, Don ; Landry, Jean Fran Çois ; Lemaire, Claude ; Miller, Jacqueline Y. ; Miller, James S. ; Miller, Lee ; Miller, Scott E. ; Montero, Jose ; Munroe, Eugene ; Green, Suzanne Rab ; Ratnasingham, Sujeevan ; Rawlins, John E. ; Robbins, Robert K. ; Rodriguez, Josephine J. ; Rougerie, Rodolphe ; Sharkey, Michael J. ; Smith, M. Alex ; Solis, M. Alma ; Sullivan, J. Bolling ; Thiaucourt, Paul ; Wahl, David B. ; Weller, Susan J. ; Whitfield, James B. ; Willmott, Keith R. ; Wood, D. Monty ; Woodley, Norman E. ; Wilson, John J. / Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity. In: Molecular Ecology Resources. 2009 ; Vol. 9, No. SUPPL. 1. pp. 1-26.
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author = "Janzen, {Daniel H.} and Winnie Hallwachs and Patrick Blandin and Burns, {John M.} and Cadiou, {Jean Marie} and Isidro Chacon and Tanya Dapkey and Deans, {Andrew Robert} and Epstein, {Marc E.} and Bernardo Espinoza and Franclemont, {John G.} and Haber, {William A.} and Mehrdad Hajibabaei and Hall, {Jason P.W.} and Hebert, {Paul D.N.} and Gauld, {Ian D.} and Harvey, {Donald J.} and Axel Hausmann and Kitching, {Ian J.} and Don Lafontaine and Landry, {Jean Fran {\cC}ois} and Claude Lemaire and Miller, {Jacqueline Y.} and Miller, {James S.} and Lee Miller and Miller, {Scott E.} and Jose Montero and Eugene Munroe and Green, {Suzanne Rab} and Sujeevan Ratnasingham and Rawlins, {John E.} and Robbins, {Robert K.} and Rodriguez, {Josephine J.} and Rodolphe Rougerie and Sharkey, {Michael J.} and Smith, {M. Alex} and Solis, {M. Alma} and Sullivan, {J. Bolling} and Paul Thiaucourt and Wahl, {David B.} and Weller, {Susan J.} and Whitfield, {James B.} and Willmott, {Keith R.} and Wood, {D. Monty} and Woodley, {Norman E.} and Wilson, {John J.}",
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Janzen, DH, Hallwachs, W, Blandin, P, Burns, JM, Cadiou, JM, Chacon, I, Dapkey, T, Deans, AR, Epstein, ME, Espinoza, B, Franclemont, JG, Haber, WA, Hajibabaei, M, Hall, JPW, Hebert, PDN, Gauld, ID, Harvey, DJ, Hausmann, A, Kitching, IJ, Lafontaine, D, Landry, JFÇ, Lemaire, C, Miller, JY, Miller, JS, Miller, L, Miller, SE, Montero, J, Munroe, E, Green, SR, Ratnasingham, S, Rawlins, JE, Robbins, RK, Rodriguez, JJ, Rougerie, R, Sharkey, MJ, Smith, MA, Solis, MA, Sullivan, JB, Thiaucourt, P, Wahl, DB, Weller, SJ, Whitfield, JB, Willmott, KR, Wood, DM, Woodley, NE & Wilson, JJ 2009, 'Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity', Molecular Ecology Resources, vol. 9, no. SUPPL. 1, pp. 1-26. https://doi.org/10.1111/j.1755-0998.2009.02628.x

Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity. / Janzen, Daniel H.; Hallwachs, Winnie; Blandin, Patrick; Burns, John M.; Cadiou, Jean Marie; Chacon, Isidro; Dapkey, Tanya; Deans, Andrew Robert; Epstein, Marc E.; Espinoza, Bernardo; Franclemont, John G.; Haber, William A.; Hajibabaei, Mehrdad; Hall, Jason P.W.; Hebert, Paul D.N.; Gauld, Ian D.; Harvey, Donald J.; Hausmann, Axel; Kitching, Ian J.; Lafontaine, Don; Landry, Jean Fran Çois; Lemaire, Claude; Miller, Jacqueline Y.; Miller, James S.; Miller, Lee; Miller, Scott E.; Montero, Jose; Munroe, Eugene; Green, Suzanne Rab; Ratnasingham, Sujeevan; Rawlins, John E.; Robbins, Robert K.; Rodriguez, Josephine J.; Rougerie, Rodolphe; Sharkey, Michael J.; Smith, M. Alex; Solis, M. Alma; Sullivan, J. Bolling; Thiaucourt, Paul; Wahl, David B.; Weller, Susan J.; Whitfield, James B.; Willmott, Keith R.; Wood, D. Monty; Woodley, Norman E.; Wilson, John J.

In: Molecular Ecology Resources, Vol. 9, No. SUPPL. 1, 01.01.2009, p. 1-26.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity

AU - Janzen, Daniel H.

AU - Hallwachs, Winnie

AU - Blandin, Patrick

AU - Burns, John M.

AU - Cadiou, Jean Marie

AU - Chacon, Isidro

AU - Dapkey, Tanya

AU - Deans, Andrew Robert

AU - Epstein, Marc E.

AU - Espinoza, Bernardo

AU - Franclemont, John G.

AU - Haber, William A.

AU - Hajibabaei, Mehrdad

AU - Hall, Jason P.W.

AU - Hebert, Paul D.N.

AU - Gauld, Ian D.

AU - Harvey, Donald J.

AU - Hausmann, Axel

AU - Kitching, Ian J.

AU - Lafontaine, Don

AU - Landry, Jean Fran Çois

AU - Lemaire, Claude

AU - Miller, Jacqueline Y.

AU - Miller, James S.

AU - Miller, Lee

AU - Miller, Scott E.

AU - Montero, Jose

AU - Munroe, Eugene

AU - Green, Suzanne Rab

AU - Ratnasingham, Sujeevan

AU - Rawlins, John E.

AU - Robbins, Robert K.

AU - Rodriguez, Josephine J.

AU - Rougerie, Rodolphe

AU - Sharkey, Michael J.

AU - Smith, M. Alex

AU - Solis, M. Alma

AU - Sullivan, J. Bolling

AU - Thiaucourt, Paul

AU - Wahl, David B.

AU - Weller, Susan J.

AU - Whitfield, James B.

AU - Willmott, Keith R.

AU - Wood, D. Monty

AU - Woodley, Norman E.

AU - Wilson, John J.

PY - 2009/1/1

Y1 - 2009/1/1

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