Integrin beta1 mediates hepatocellular carcinoma cells chemotaxis to laminin

Bian hong Fu, Ze zhi Wu, Hai yan Zhang, Jian Qin, Shao xi Cai, Yun peng Wu, Cheng Dong

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To study the effects of integrin beta1 on the chemotaxis of hepatocellular carcinoma (HCC) cells to laminin (LN). METHODS: A micropipette technique was adopted to investigate the effect of integrin beta1 blockade on pseudopod protrusion of HCC cells in response to LN stimulation. Chemotactic pseudopod protrusion of a HCC cell was evaluated using a dual-pipette set-up, in which two pipettes filled with LN solution were positional in close contact with the same cell, and pseudopod protrusion into each pipette was viewed dynamically and recorded with a tape recorder. The lengths of pseudopods were measured, then plotted against time to obtain a pseudopod growth curve. The integrin beta1 subunit on the surfaces of HCC cells was analyzed by flow cytometry. RESULTS: In dual pipette chemotaxis experiment, when the two pipettes were filled with LN (50microg/ml, 200microg/ml), pseudopods extended from the HCC cells into each of the pipettes nearly symmetrically. Upon addition of anti-CD29 (20microg/ml) to one of the pipettes, the pseudopod protrusion was blocked almost completely, while the pseudopod protrusion into the opposite pipette became more evidently, with larger maximum length. The expression rate of integrin beta1 on the cells was up to 95.78%. CONCLUSION: Integrin beta1 subunit is the important receptor for mediating HCC cells chemotaxis to laminin.

Original languageEnglish (US)
Pages (from-to)605-608
Number of pages4
JournalZhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Volume11
Issue number10
StatePublished - Jan 1 2003

Fingerprint

CD29 Antigens
Pseudopodia
Laminin
Chemotaxis
Hepatocellular Carcinoma
Flow Cytometry

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Fu, Bian hong ; Wu, Ze zhi ; Zhang, Hai yan ; Qin, Jian ; Cai, Shao xi ; Wu, Yun peng ; Dong, Cheng. / Integrin beta1 mediates hepatocellular carcinoma cells chemotaxis to laminin. In: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology. 2003 ; Vol. 11, No. 10. pp. 605-608.
@article{f4fb27ceec7d4549bf8f0310af8b86d3,
title = "Integrin beta1 mediates hepatocellular carcinoma cells chemotaxis to laminin",
abstract = "OBJECTIVE: To study the effects of integrin beta1 on the chemotaxis of hepatocellular carcinoma (HCC) cells to laminin (LN). METHODS: A micropipette technique was adopted to investigate the effect of integrin beta1 blockade on pseudopod protrusion of HCC cells in response to LN stimulation. Chemotactic pseudopod protrusion of a HCC cell was evaluated using a dual-pipette set-up, in which two pipettes filled with LN solution were positional in close contact with the same cell, and pseudopod protrusion into each pipette was viewed dynamically and recorded with a tape recorder. The lengths of pseudopods were measured, then plotted against time to obtain a pseudopod growth curve. The integrin beta1 subunit on the surfaces of HCC cells was analyzed by flow cytometry. RESULTS: In dual pipette chemotaxis experiment, when the two pipettes were filled with LN (50microg/ml, 200microg/ml), pseudopods extended from the HCC cells into each of the pipettes nearly symmetrically. Upon addition of anti-CD29 (20microg/ml) to one of the pipettes, the pseudopod protrusion was blocked almost completely, while the pseudopod protrusion into the opposite pipette became more evidently, with larger maximum length. The expression rate of integrin beta1 on the cells was up to 95.78{\%}. CONCLUSION: Integrin beta1 subunit is the important receptor for mediating HCC cells chemotaxis to laminin.",
author = "Fu, {Bian hong} and Wu, {Ze zhi} and Zhang, {Hai yan} and Jian Qin and Cai, {Shao xi} and Wu, {Yun peng} and Cheng Dong",
year = "2003",
month = "1",
day = "1",
language = "English (US)",
volume = "11",
pages = "605--608",
journal = "Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology",
issn = "1007-3418",
publisher = "Zhonghua Yixuehui Zazhishe",
number = "10",

}

Integrin beta1 mediates hepatocellular carcinoma cells chemotaxis to laminin. / Fu, Bian hong; Wu, Ze zhi; Zhang, Hai yan; Qin, Jian; Cai, Shao xi; Wu, Yun peng; Dong, Cheng.

In: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology, Vol. 11, No. 10, 01.01.2003, p. 605-608.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Integrin beta1 mediates hepatocellular carcinoma cells chemotaxis to laminin

AU - Fu, Bian hong

AU - Wu, Ze zhi

AU - Zhang, Hai yan

AU - Qin, Jian

AU - Cai, Shao xi

AU - Wu, Yun peng

AU - Dong, Cheng

PY - 2003/1/1

Y1 - 2003/1/1

N2 - OBJECTIVE: To study the effects of integrin beta1 on the chemotaxis of hepatocellular carcinoma (HCC) cells to laminin (LN). METHODS: A micropipette technique was adopted to investigate the effect of integrin beta1 blockade on pseudopod protrusion of HCC cells in response to LN stimulation. Chemotactic pseudopod protrusion of a HCC cell was evaluated using a dual-pipette set-up, in which two pipettes filled with LN solution were positional in close contact with the same cell, and pseudopod protrusion into each pipette was viewed dynamically and recorded with a tape recorder. The lengths of pseudopods were measured, then plotted against time to obtain a pseudopod growth curve. The integrin beta1 subunit on the surfaces of HCC cells was analyzed by flow cytometry. RESULTS: In dual pipette chemotaxis experiment, when the two pipettes were filled with LN (50microg/ml, 200microg/ml), pseudopods extended from the HCC cells into each of the pipettes nearly symmetrically. Upon addition of anti-CD29 (20microg/ml) to one of the pipettes, the pseudopod protrusion was blocked almost completely, while the pseudopod protrusion into the opposite pipette became more evidently, with larger maximum length. The expression rate of integrin beta1 on the cells was up to 95.78%. CONCLUSION: Integrin beta1 subunit is the important receptor for mediating HCC cells chemotaxis to laminin.

AB - OBJECTIVE: To study the effects of integrin beta1 on the chemotaxis of hepatocellular carcinoma (HCC) cells to laminin (LN). METHODS: A micropipette technique was adopted to investigate the effect of integrin beta1 blockade on pseudopod protrusion of HCC cells in response to LN stimulation. Chemotactic pseudopod protrusion of a HCC cell was evaluated using a dual-pipette set-up, in which two pipettes filled with LN solution were positional in close contact with the same cell, and pseudopod protrusion into each pipette was viewed dynamically and recorded with a tape recorder. The lengths of pseudopods were measured, then plotted against time to obtain a pseudopod growth curve. The integrin beta1 subunit on the surfaces of HCC cells was analyzed by flow cytometry. RESULTS: In dual pipette chemotaxis experiment, when the two pipettes were filled with LN (50microg/ml, 200microg/ml), pseudopods extended from the HCC cells into each of the pipettes nearly symmetrically. Upon addition of anti-CD29 (20microg/ml) to one of the pipettes, the pseudopod protrusion was blocked almost completely, while the pseudopod protrusion into the opposite pipette became more evidently, with larger maximum length. The expression rate of integrin beta1 on the cells was up to 95.78%. CONCLUSION: Integrin beta1 subunit is the important receptor for mediating HCC cells chemotaxis to laminin.

UR - http://www.scopus.com/inward/record.url?scp=21244464430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21244464430&partnerID=8YFLogxK

M3 - Article

C2 - 14572338

AN - SCOPUS:21244464430

VL - 11

SP - 605

EP - 608

JO - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology

JF - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology

SN - 1007-3418

IS - 10

ER -