TY - JOUR
T1 - Interaction of nanogel with cyclodextrin or protein
T2 - Study by dynamic light scattering and small-angle neutron scattering
AU - Inomoto, Norihiro
AU - Osaka, Noboru
AU - Suzuki, Takuya
AU - Hasegawa, Urara
AU - Ozawa, Yayoi
AU - Endo, Hitoshi
AU - Akiyoshi, Kazunari
AU - Shibayama, Mitsuhiro
N1 - Funding Information:
This work was also partially supported by the Ministry of Education, Science, Sports and Culture, Japan (Grant-in-Aid for Scientific Research (A), 2006–2008, No. 18205025, and for Scientific Research on Priority Areas, 2006–2010, No. 18068004). The SANS experiment was performed with the approval of Institute for Solid State Physics, The University of Tokyo (Proposal Nos. 06.237), at Japan Atomic Energy Agency, Tokai, Japan.
PY - 2009/1/16
Y1 - 2009/1/16
N2 - The structure of hydrogel nanoparticles (CHP nanogels), formed by self-aggregation of cholesterol-bearing pullulan (CHP) was studied by dynamic light scattering (DLS) and small-angle neutron scattering (SANS). The interactions between the CHP nanogel and methyl-β-cyclodextrin (CD) or protein (hen egg white) were also investigated. It was revealed by SANS that the nanogels were spherical in shape with a radius of 6.7 nm. The following two functions were disclosed. (1) CHP nanogels were dissociated by the addition of CD and formed inclusion complexes with cholesteryl groups, leading to suppression of hydrophobic interaction between the cholesteryl groups. (2) The nanogel behaved as a molecular chaperone (heat shock protein-like activity) when CHP nanogel was mixed with hen egg white and heated up to 75 °C. The egg white aqueous solutions with CHP nanogel remained transparent while the egg white without CHP nanogel became opaque.
AB - The structure of hydrogel nanoparticles (CHP nanogels), formed by self-aggregation of cholesterol-bearing pullulan (CHP) was studied by dynamic light scattering (DLS) and small-angle neutron scattering (SANS). The interactions between the CHP nanogel and methyl-β-cyclodextrin (CD) or protein (hen egg white) were also investigated. It was revealed by SANS that the nanogels were spherical in shape with a radius of 6.7 nm. The following two functions were disclosed. (1) CHP nanogels were dissociated by the addition of CD and formed inclusion complexes with cholesteryl groups, leading to suppression of hydrophobic interaction between the cholesteryl groups. (2) The nanogel behaved as a molecular chaperone (heat shock protein-like activity) when CHP nanogel was mixed with hen egg white and heated up to 75 °C. The egg white aqueous solutions with CHP nanogel remained transparent while the egg white without CHP nanogel became opaque.
UR - http://www.scopus.com/inward/record.url?scp=58149191322&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149191322&partnerID=8YFLogxK
U2 - 10.1016/j.polymer.2008.11.001
DO - 10.1016/j.polymer.2008.11.001
M3 - Article
AN - SCOPUS:58149191322
VL - 50
SP - 541
EP - 546
JO - Polymer
JF - Polymer
SN - 0032-3861
IS - 2
ER -