Interaction of P-glycoprotein with protein kinase C in human multidrug resistant carcinoma cells

Jin-Ming Yang, Khew Voon Chin, William N. Hait

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Abstract

Indirect evidence has suggested that P-glycoprotein (P-gp), the multidrug transporter, is phosphorylated by protein kinase C (PKC) and that phosphorylation modulates its transport function. To address the first premise more directly, i.e., that P-gp is phosphorylated by PKC, we investigated the interaction between P-gp and PKC in sensitive and multidrug resistant MCF-7 and KB human carcinoma cell lines. We found that P-gp and PKC were coimmunoprecipitated from the multidrug-resistant cell lines MCF-7/AdrR and KB-V-1, using antibodies to either protein. The association between the two proteins was enhanced by phorbol 12-myristate 13-acetate, an analogue of diacylglycerol that induces translocation of PKC to the plasma membrane. The anti-P-gp immunoprecipitates contained PKC activity as measured by direct phosphorylation reactions. The interaction of PKC with P-gp displayed isozyme specificity: PKC-α, -β, -γ -ε, and -θ, but not -δ, -μ, -ζ, and -λ, were found to coimmunoprecipitate with P-gp. These studies indicate that P- gp closely interacts with PKC and serves as a substrate, and that specific isozymes of this kinase may be involved in the phosphorylation of the multidrug transporter.

Original languageEnglish (US)
Pages (from-to)3490-3494
Number of pages5
JournalCancer Research
Volume56
Issue number15
StatePublished - Aug 1 1996

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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