Interactions of the cytoplasmic domain of sindbis virus E2 with nucleocapsid cores promote alphavirus budding

Joyce Jose, Laralynne Przybyla, Thomas J. Edwards, Rushika Perera, John W. Burgner, Richard J. Kuhn

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Alphavirus budding from the plasma membrane occurs through the specific interaction of the nucleocapsid core with the cytoplasmic domain of the E2 glycoprotein (cdE2). Structural studies of the Sindbis virus capsid protein (CP) have suggested that these critical interactions are mediated by the binding of cdE2 into a hydrophobic pocket in the CP. Several molecular genetic studies have implicated amino acids Y400 and L402 in cdE2 as important for the budding of alphaviruses. In this study, we characterized the role of cdE2 residues in structural polyprotein processing, glycoprotein transport, and capsid interactions. Along with hydrophobic residues, charged residues in the N terminus of cdE2 were critical for the effective interaction of cores with cdE2, a process required for virus budding. Mutations in the C-terminal signal sequence region of cdE2 affected E2 protein transport to the plasma membrane, while nonbudding mutants that were defective in cdE2-CP interaction accumulated E2 on the plasma membrane. The interaction of cdE2 with cytoplasmic cores purified from infected cells and in vitro-assembled corelike particles suggests that cdE2 interacts with assembled cores to mediate budding. We hypothesize that these cdE2 interactions induce a change in the organization of the nucleocapsid core upon binding leading to particle budding and priming of the nucleocapsid cores for disassembly that is required for virus infection.

Original languageEnglish (US)
Pages (from-to)2585-2599
Number of pages15
JournalJournal of virology
Volume86
Issue number5
DOIs
StatePublished - Mar 1 2012

Fingerprint

Sindbis virus
Sindbis Virus
Alphavirus
Nucleocapsid
nucleocapsid
glycoproteins
Glycoproteins
Capsid Proteins
coat proteins
plasma membrane
Cell Membrane
Virus Release
Polyproteins
viruses
capsid
protein transport
Capsid
Protein Transport
Virus Diseases
Protein Sorting Signals

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Jose, Joyce ; Przybyla, Laralynne ; Edwards, Thomas J. ; Perera, Rushika ; Burgner, John W. ; Kuhn, Richard J. / Interactions of the cytoplasmic domain of sindbis virus E2 with nucleocapsid cores promote alphavirus budding. In: Journal of virology. 2012 ; Vol. 86, No. 5. pp. 2585-2599.
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Interactions of the cytoplasmic domain of sindbis virus E2 with nucleocapsid cores promote alphavirus budding. / Jose, Joyce; Przybyla, Laralynne; Edwards, Thomas J.; Perera, Rushika; Burgner, John W.; Kuhn, Richard J.

In: Journal of virology, Vol. 86, No. 5, 01.03.2012, p. 2585-2599.

Research output: Contribution to journalArticle

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