Interleukin-18 promotes sleep in rabbits and rats

Takeshi Kubota, Jidong Fang, Richard A. Brown, James M. Krueger

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Interleukin (IL)-1β is involved in physiological sleep regulation. IL-18 is a member of the IL-1 family, and its signal-transduction mechanism is similar to that of IL-1. Therefore, we hypothesized that IL-18 might also be involved in sleep regulation. Three doses of IL-18 (10, 100, and 500 ng) were injected intracerebroventricularly (icv) into rabbits at the onset of the dark period. The two higher doses of IL-18 markedly increased non-rapid eye movement sleep (NREMS), accompanied by increases in brain temperature (Tbr). These effects were lost after the heat inactivation of IL-18. The 500 ng of IL-18 injection during the light period also increased NREMS and Tbr. Similar results were obtained after icv injection of 100 ng of IL-18 into rats. Furthermore, intraperitoneal injection of 30 μg/kg of IL-18 slightly, but significantly, increased NREMS, whereas it significantly decreased electroencephalogram slow-wave activity in rats. Intraperitoneal IL-18 failed to induce fever. An anti-human IL-18 antibody had little effect on spontaneous sleep in rabbits, although the anti-IL-18 antibody significantly attenuated muramyl dipeptide-induced sleep. These data suggest that IL-18 is involved in mechanisms of sleep responses to infection.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume281
Issue number3 50-3
StatePublished - Oct 2 2001

Fingerprint

Interleukin-18
Sleep
Rabbits
Eye Movements
Interleukin-1
Acetylmuramyl-Alanyl-Isoglutamine
Injections
Antibodies
Intraperitoneal Injections
Interleukin-10
Electroencephalography
Signal Transduction
Fever
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

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Interleukin-18 promotes sleep in rabbits and rats. / Kubota, Takeshi; Fang, Jidong; Brown, Richard A.; Krueger, James M.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 281, No. 3 50-3, 02.10.2001.

Research output: Contribution to journalArticle

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