Interplay of TBP inhibitors in global transcriptional control

Carmelata Chitikila, Kathryn L. Huisinga, Jordan D. Irvin, Andrew D. Basehoar, B. Franklin Pugh

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The TATA binding protein (TBP) is required for the expression of nearly all genes and is highly regulated both positively and negatively. Here, we use DNA microarrays to explore the genome-wide interplay of several TBP-interacting inhibitors in the yeast Saccharomyces cerevisiae. Our findings suggest the following: The NC2 inhibitor turns down, but not off, highly active genes. Autoinhibition of TBP through dimerization contributes to transcriptional repression, even at repressive subtelomeric regions. The TAND domain of TAF1 plays a primary inhibitory role at very few genes, but its function becomes widespread when other TBP interactions are compromised. These findings reveal that transcriptional output is limited in part by a collaboration of different combinations of TBP inhibitory mechanisms.

Original languageEnglish (US)
Pages (from-to)871-882
Number of pages12
JournalMolecular cell
Volume10
Issue number4
DOIs
StatePublished - Oct 1 2002

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Interplay of TBP inhibitors in global transcriptional control'. Together they form a unique fingerprint.

  • Cite this

    Chitikila, C., Huisinga, K. L., Irvin, J. D., Basehoar, A. D., & Pugh, B. F. (2002). Interplay of TBP inhibitors in global transcriptional control. Molecular cell, 10(4), 871-882. https://doi.org/10.1016/S1097-2765(02)00683-4