Interrogating Canonical Wnt Signaling Pathway in Human Pluripotent Stem Cell Fate Decisions Using CRISPR-Cas9

Xiaojun Lian, Jiejia Xu, Xiaoping Bao, Lauren N. Randolph

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Human pluripotent stem cells (hPSCs) offer tremendous promise in tissue engineering and cell-based therapies because of their unique combination of two properties: pluripotency and virtually unlimited proliferative potential. Currently, a lack of efficient hPSC differentiation methods impedes the use of hPSCs in biomedical translational research. To overcome this hurdle, one important prerequisite is to understand how hPSCs integrate environmental signaling cues to regulate fate choices. Wnt/β-catenin is a critical signaling pathway participating in stem cell maintenance and differentiation; however the exact role of Wnt/β-catenin signaling in hPSCs fate decisions remains controversial. Here, we engineered a single guide RNA (sgRNA) and Cas9-GFP system to overcome the low genome editing efficiency in hPSCs and achieved extremely high β-catenin knockout efficiency (up to 25%), demonstrating the robust nature of this system for hPSC genome editing. To the best of our knowledge, this represents the first reported β-catenin knockout hPSCs. Furthermore, we found that Wnt/β-catenin signaling is not required for hPSCs expansion or neuroectoderm differentiation. However, Wnt/β-catenin signaling is absolutely required to generate mesendoderm lineage which includes cardiomyocyte differentiation. The robust ability to generate gene knockout hPSC lines using the sgRNA and Cas9-GFP system should facilitate future study of gene functions in stem cell research.

Original languageEnglish (US)
Pages (from-to)325-334
Number of pages10
JournalCellular and Molecular Bioengineering
Volume9
Issue number3
DOIs
StatePublished - Sep 1 2016

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Pluripotent Stem Cells
Wnt Signaling Pathway
Stem Cells
Signaling Pathways
Stem cells
Catenins
Guide RNA
Genes
Cell Differentiation
RNA
Human
Genome
Neural Plate
Efficiency
Stem Cell Research
Gene Knockout Techniques
Aptitude
Critical Pathways
Translational Medical Research

All Science Journal Classification (ASJC) codes

  • Modeling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{4eecc66c64254dd6a1fe99f024b66fb0,
title = "Interrogating Canonical Wnt Signaling Pathway in Human Pluripotent Stem Cell Fate Decisions Using CRISPR-Cas9",
abstract = "Human pluripotent stem cells (hPSCs) offer tremendous promise in tissue engineering and cell-based therapies because of their unique combination of two properties: pluripotency and virtually unlimited proliferative potential. Currently, a lack of efficient hPSC differentiation methods impedes the use of hPSCs in biomedical translational research. To overcome this hurdle, one important prerequisite is to understand how hPSCs integrate environmental signaling cues to regulate fate choices. Wnt/β-catenin is a critical signaling pathway participating in stem cell maintenance and differentiation; however the exact role of Wnt/β-catenin signaling in hPSCs fate decisions remains controversial. Here, we engineered a single guide RNA (sgRNA) and Cas9-GFP system to overcome the low genome editing efficiency in hPSCs and achieved extremely high β-catenin knockout efficiency (up to 25{\%}), demonstrating the robust nature of this system for hPSC genome editing. To the best of our knowledge, this represents the first reported β-catenin knockout hPSCs. Furthermore, we found that Wnt/β-catenin signaling is not required for hPSCs expansion or neuroectoderm differentiation. However, Wnt/β-catenin signaling is absolutely required to generate mesendoderm lineage which includes cardiomyocyte differentiation. The robust ability to generate gene knockout hPSC lines using the sgRNA and Cas9-GFP system should facilitate future study of gene functions in stem cell research.",
author = "Xiaojun Lian and Jiejia Xu and Xiaoping Bao and Randolph, {Lauren N.}",
year = "2016",
month = "9",
day = "1",
doi = "10.1007/s12195-016-0453-8",
language = "English (US)",
volume = "9",
pages = "325--334",
journal = "Cellular and Molecular Bioengineering",
issn = "1865-5025",
publisher = "Springer New York",
number = "3",

}

Interrogating Canonical Wnt Signaling Pathway in Human Pluripotent Stem Cell Fate Decisions Using CRISPR-Cas9. / Lian, Xiaojun; Xu, Jiejia; Bao, Xiaoping; Randolph, Lauren N.

In: Cellular and Molecular Bioengineering, Vol. 9, No. 3, 01.09.2016, p. 325-334.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Interrogating Canonical Wnt Signaling Pathway in Human Pluripotent Stem Cell Fate Decisions Using CRISPR-Cas9

AU - Lian, Xiaojun

AU - Xu, Jiejia

AU - Bao, Xiaoping

AU - Randolph, Lauren N.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Human pluripotent stem cells (hPSCs) offer tremendous promise in tissue engineering and cell-based therapies because of their unique combination of two properties: pluripotency and virtually unlimited proliferative potential. Currently, a lack of efficient hPSC differentiation methods impedes the use of hPSCs in biomedical translational research. To overcome this hurdle, one important prerequisite is to understand how hPSCs integrate environmental signaling cues to regulate fate choices. Wnt/β-catenin is a critical signaling pathway participating in stem cell maintenance and differentiation; however the exact role of Wnt/β-catenin signaling in hPSCs fate decisions remains controversial. Here, we engineered a single guide RNA (sgRNA) and Cas9-GFP system to overcome the low genome editing efficiency in hPSCs and achieved extremely high β-catenin knockout efficiency (up to 25%), demonstrating the robust nature of this system for hPSC genome editing. To the best of our knowledge, this represents the first reported β-catenin knockout hPSCs. Furthermore, we found that Wnt/β-catenin signaling is not required for hPSCs expansion or neuroectoderm differentiation. However, Wnt/β-catenin signaling is absolutely required to generate mesendoderm lineage which includes cardiomyocyte differentiation. The robust ability to generate gene knockout hPSC lines using the sgRNA and Cas9-GFP system should facilitate future study of gene functions in stem cell research.

AB - Human pluripotent stem cells (hPSCs) offer tremendous promise in tissue engineering and cell-based therapies because of their unique combination of two properties: pluripotency and virtually unlimited proliferative potential. Currently, a lack of efficient hPSC differentiation methods impedes the use of hPSCs in biomedical translational research. To overcome this hurdle, one important prerequisite is to understand how hPSCs integrate environmental signaling cues to regulate fate choices. Wnt/β-catenin is a critical signaling pathway participating in stem cell maintenance and differentiation; however the exact role of Wnt/β-catenin signaling in hPSCs fate decisions remains controversial. Here, we engineered a single guide RNA (sgRNA) and Cas9-GFP system to overcome the low genome editing efficiency in hPSCs and achieved extremely high β-catenin knockout efficiency (up to 25%), demonstrating the robust nature of this system for hPSC genome editing. To the best of our knowledge, this represents the first reported β-catenin knockout hPSCs. Furthermore, we found that Wnt/β-catenin signaling is not required for hPSCs expansion or neuroectoderm differentiation. However, Wnt/β-catenin signaling is absolutely required to generate mesendoderm lineage which includes cardiomyocyte differentiation. The robust ability to generate gene knockout hPSC lines using the sgRNA and Cas9-GFP system should facilitate future study of gene functions in stem cell research.

UR - http://www.scopus.com/inward/record.url?scp=84973597822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973597822&partnerID=8YFLogxK

U2 - 10.1007/s12195-016-0453-8

DO - 10.1007/s12195-016-0453-8

M3 - Article

AN - SCOPUS:84973597822

VL - 9

SP - 325

EP - 334

JO - Cellular and Molecular Bioengineering

JF - Cellular and Molecular Bioengineering

SN - 1865-5025

IS - 3

ER -