TY - JOUR
T1 - Interval Exercise Lowers Circulating CD105 Extracellular Vesicles in Prediabetes
AU - Eichner, Natalie Z.M.
AU - Gilbertson, Nicole M.
AU - Heiston, Emily M.
AU - Musante, Luca
AU - La Salvia, Sabrina
AU - Weltman, Arthur
AU - Erdbrugger, Uta
AU - Malin, Steven K.
N1 - Funding Information:
The authors would like to thank Dr. Eugene J. Barrett, MD, PhD for his assistance in screening subjects and helpful feedback on the work. The authors also thank the members of the Applied Metabolism & Physiology Laboratory for help on the study, particularly Julian M. Gaitán. The authors also thank the clinical research unit nursing staff, the exercise physiology core laboratory staff, and participants for their excellent efforts. Funding was supported by the University of Virginia’s Curry School of Education (S. K. M.) and Launchpad Diabetes Award (S. K. M. and U. E.). S. K. M. is supported by National Institutes of Health RO1-HL130296.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Background Extracellular vesicles (EV) are purported to mediate type 2 diabetes and CVD risk and development. Physical activity and a balanced diet reduce disease risk, but no study has tested the hypothesis that short-term interval (INT) training would reduce EV compared with continuous (CONT) exercise in adults with prediabetes. Methods Eighteen obese adults (age, 63.8 ± 1.5 yr; body mass index, 31.0 ± 1.3 kg·m-2) were screened for prediabetes using American Diabetes Association criteria (75 g oral glucose tolerance test). Subjects were randomized to INT (n = 10, alternating 3-min intervals at 90% and 50% HRpeak, respectively) or CONT (n = 8, 70% HRpeak) training for 12 supervised sessions over 13 d for 60 min·d-1. Cardiorespiratory fitness (VO2peak), weight (kg), as well as ad libitum dietary intake were assessed and arterial stiffness (augmentation index via applanation tonometry) was calculated using total AUC during a 75-g oral glucose tolerance test performed 24 h after the last exercise bout. Total EV, platelet EV (CD31+/CD41+), endothelial EV (CD105; CD31+/ CD41-), platelet endothelial cell adhesion molecule (PECAM) (CD31+), and leukocyte EV (CD45+; CD45+/CD41-) were analyzed via imaging flow cytometry preintervention/postintervention. Results The INT exercise increased VO2peak (P = 0.04) compared with CONT training. Although training had no effect on platelet or leukocyte EV, INT decreased Annexin V- endothelial EV CD105 compared with CONT (P = 0.04). However, after accounting for dietary sugar intake, the intensity effect was lost (P = 0.18). Increased ad libitum dietary sugar intake after training was linked to elevated AV+ CD105 (r = 0.49, P = 0.06) and AV- CD45+ (r = 0.59, P = 0.01). Nonetheless, increased VO2peak correlated with decreased AV+ CD105 (r = -0.60, P = 0.01). Conclusions Interval exercise training decreases endothelial-derived EV in adults with prediabetes. Although increased sugar consumption may alter EV after a short-term exercise intervention, fitness modifies EV count.
AB - Background Extracellular vesicles (EV) are purported to mediate type 2 diabetes and CVD risk and development. Physical activity and a balanced diet reduce disease risk, but no study has tested the hypothesis that short-term interval (INT) training would reduce EV compared with continuous (CONT) exercise in adults with prediabetes. Methods Eighteen obese adults (age, 63.8 ± 1.5 yr; body mass index, 31.0 ± 1.3 kg·m-2) were screened for prediabetes using American Diabetes Association criteria (75 g oral glucose tolerance test). Subjects were randomized to INT (n = 10, alternating 3-min intervals at 90% and 50% HRpeak, respectively) or CONT (n = 8, 70% HRpeak) training for 12 supervised sessions over 13 d for 60 min·d-1. Cardiorespiratory fitness (VO2peak), weight (kg), as well as ad libitum dietary intake were assessed and arterial stiffness (augmentation index via applanation tonometry) was calculated using total AUC during a 75-g oral glucose tolerance test performed 24 h after the last exercise bout. Total EV, platelet EV (CD31+/CD41+), endothelial EV (CD105; CD31+/ CD41-), platelet endothelial cell adhesion molecule (PECAM) (CD31+), and leukocyte EV (CD45+; CD45+/CD41-) were analyzed via imaging flow cytometry preintervention/postintervention. Results The INT exercise increased VO2peak (P = 0.04) compared with CONT training. Although training had no effect on platelet or leukocyte EV, INT decreased Annexin V- endothelial EV CD105 compared with CONT (P = 0.04). However, after accounting for dietary sugar intake, the intensity effect was lost (P = 0.18). Increased ad libitum dietary sugar intake after training was linked to elevated AV+ CD105 (r = 0.49, P = 0.06) and AV- CD45+ (r = 0.59, P = 0.01). Nonetheless, increased VO2peak correlated with decreased AV+ CD105 (r = -0.60, P = 0.01). Conclusions Interval exercise training decreases endothelial-derived EV in adults with prediabetes. Although increased sugar consumption may alter EV after a short-term exercise intervention, fitness modifies EV count.
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U2 - 10.1249/MSS.0000000000002185
DO - 10.1249/MSS.0000000000002185
M3 - Article
C2 - 31609300
AN - SCOPUS:85079344257
VL - 52
SP - 729
EP - 735
JO - Medicine and Science in Sports and Exercise
JF - Medicine and Science in Sports and Exercise
SN - 0195-9131
IS - 3
ER -