Interval Exercise Lowers Circulating CD105 Extracellular Vesicles in Prediabetes

Natalie Z.M. Eichner, Nicole M. Gilbertson, Emily M. Heiston, Luca Musante, Sabrina La Salvia, Arthur Weltman, Uta Erdbrugger, Steven K. Malin

Research output: Contribution to journalArticlepeer-review

Abstract

Background Extracellular vesicles (EV) are purported to mediate type 2 diabetes and CVD risk and development. Physical activity and a balanced diet reduce disease risk, but no study has tested the hypothesis that short-term interval (INT) training would reduce EV compared with continuous (CONT) exercise in adults with prediabetes. Methods Eighteen obese adults (age, 63.8 ± 1.5 yr; body mass index, 31.0 ± 1.3 kg·m-2) were screened for prediabetes using American Diabetes Association criteria (75 g oral glucose tolerance test). Subjects were randomized to INT (n = 10, alternating 3-min intervals at 90% and 50% HRpeak, respectively) or CONT (n = 8, 70% HRpeak) training for 12 supervised sessions over 13 d for 60 min·d-1. Cardiorespiratory fitness (VO2peak), weight (kg), as well as ad libitum dietary intake were assessed and arterial stiffness (augmentation index via applanation tonometry) was calculated using total AUC during a 75-g oral glucose tolerance test performed 24 h after the last exercise bout. Total EV, platelet EV (CD31+/CD41+), endothelial EV (CD105; CD31+/ CD41-), platelet endothelial cell adhesion molecule (PECAM) (CD31+), and leukocyte EV (CD45+; CD45+/CD41-) were analyzed via imaging flow cytometry preintervention/postintervention. Results The INT exercise increased VO2peak (P = 0.04) compared with CONT training. Although training had no effect on platelet or leukocyte EV, INT decreased Annexin V- endothelial EV CD105 compared with CONT (P = 0.04). However, after accounting for dietary sugar intake, the intensity effect was lost (P = 0.18). Increased ad libitum dietary sugar intake after training was linked to elevated AV+ CD105 (r = 0.49, P = 0.06) and AV- CD45+ (r = 0.59, P = 0.01). Nonetheless, increased VO2peak correlated with decreased AV+ CD105 (r = -0.60, P = 0.01). Conclusions Interval exercise training decreases endothelial-derived EV in adults with prediabetes. Although increased sugar consumption may alter EV after a short-term exercise intervention, fitness modifies EV count.

Original languageEnglish (US)
Pages (from-to)729-735
Number of pages7
JournalMedicine and science in sports and exercise
Volume52
Issue number3
DOIs
StatePublished - Mar 1 2020

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

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