TY - JOUR
T1 - Intracellular Delivery of Gold Nanocolloids Promoted by a Chemically Conjugated Anticancer Peptide
AU - Kapur, Anshika
AU - Medina, Scott H.
AU - Wang, Wentao
AU - Palui, Goutam
AU - Schneider, Joel P.
AU - Mattoussi, Hedi
N1 - Funding Information:
This work was supported by the National Science Foundation (NSF-CHE #1508501), National Institutes of Health (NIH #R01 DC013080), Asahi-Kasei Corp., and the Intramural Research Program of the National Cancer Institute (National Institutes of Health, Project #BC011313).
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/10/31
Y1 - 2018/10/31
N2 - We report on the ability of a chemically synthesized anticancer peptide, SVS-1, to promote the rapid uptake of gold nanorods (AuNRs) and gold nanoparticles (AuNPs) by live HeLa cells. For this, AuNPs and AuNRs, surface ligated with a multicoordinating polymer that presents several amine groups per ligand, are simultaneously reacted with SVS-1 and Texas-Red dye; the latter allows fluorescence visualization of the nanocrystals. Using epifluorescence microscopy, we find that incubation of the SVS-1-conjugated AuNPs and AuNRs with a model cancer cell line yields extended staining throughout the cell cytoplasm, even at low conjugate concentrations (∼0.1 nM). Furthermore, uptake is specific to the SVS-1-conjugated nanocrystals. Additional endocytosis inhibition experiments, where cells have been incubated with the conjugates at 4 °C or in the presence of endocytic inhibitors, show that significant levels of conjugate uptake persist. These results combined indicate an uptake mechanism that does not necessarily rely on endocytosis, a promising finding with implications for the use of nanomaterials in the field of biology and nanomedicine.
AB - We report on the ability of a chemically synthesized anticancer peptide, SVS-1, to promote the rapid uptake of gold nanorods (AuNRs) and gold nanoparticles (AuNPs) by live HeLa cells. For this, AuNPs and AuNRs, surface ligated with a multicoordinating polymer that presents several amine groups per ligand, are simultaneously reacted with SVS-1 and Texas-Red dye; the latter allows fluorescence visualization of the nanocrystals. Using epifluorescence microscopy, we find that incubation of the SVS-1-conjugated AuNPs and AuNRs with a model cancer cell line yields extended staining throughout the cell cytoplasm, even at low conjugate concentrations (∼0.1 nM). Furthermore, uptake is specific to the SVS-1-conjugated nanocrystals. Additional endocytosis inhibition experiments, where cells have been incubated with the conjugates at 4 °C or in the presence of endocytic inhibitors, show that significant levels of conjugate uptake persist. These results combined indicate an uptake mechanism that does not necessarily rely on endocytosis, a promising finding with implications for the use of nanomaterials in the field of biology and nanomedicine.
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U2 - 10.1021/acsomega.8b02276
DO - 10.1021/acsomega.8b02276
M3 - Article
C2 - 30411018
AN - SCOPUS:85054574259
VL - 3
SP - 12754
EP - 12762
JO - ACS Omega
JF - ACS Omega
SN - 2470-1343
IS - 10
ER -