Intracellular localization of the tumor suppressor HtrA1/Prss11 and its association with HPV16 E6 and E7 proteins

Gary Clawson, Vuong Bui, Ping Xin, Ning Wang, Weihua Pan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have a long-standing interest in a nuclear protease which appears to be involved in carcinogenesis. We recently identified the protease as high temperature requirement factor A 1 (HtrA1), also known as Prss11, which is member of an oxidative stress-response family of proteases. HtrA1 has been classified as a secreted protease involved in TGFβ signaling, but recent work has shown HtrA1 to be a tumor suppressor. Here we show that processed forms of HtrA1 are found intracellularly and intranuclearly, and the active intranuclear form of HtrA1 shows an ∼Mr 29,000. Further, expression of HPV E6/E7 proteins is associated with a post-transcriptional up-regulation of HtrA1 (most notably the nuclear form), and HtrA1 is found associated with both HPV E6 and E7 proteins.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalJournal of Cellular Biochemistry
Volume105
Issue number1
DOIs
StatePublished - Sep 1 2008

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Tumors
Association reactions
Temperature
Peptide Hydrolases
Neoplasms
Proteins
Oxidative stress
oncogene protein E7, Human papillomavirus type 16
Carcinogenesis
Oxidative Stress
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "We have a long-standing interest in a nuclear protease which appears to be involved in carcinogenesis. We recently identified the protease as high temperature requirement factor A 1 (HtrA1), also known as Prss11, which is member of an oxidative stress-response family of proteases. HtrA1 has been classified as a secreted protease involved in TGFβ signaling, but recent work has shown HtrA1 to be a tumor suppressor. Here we show that processed forms of HtrA1 are found intracellularly and intranuclearly, and the active intranuclear form of HtrA1 shows an ∼Mr 29,000. Further, expression of HPV E6/E7 proteins is associated with a post-transcriptional up-regulation of HtrA1 (most notably the nuclear form), and HtrA1 is found associated with both HPV E6 and E7 proteins.",
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Intracellular localization of the tumor suppressor HtrA1/Prss11 and its association with HPV16 E6 and E7 proteins. / Clawson, Gary; Bui, Vuong; Xin, Ping; Wang, Ning; Pan, Weihua.

In: Journal of Cellular Biochemistry, Vol. 105, No. 1, 01.09.2008, p. 81-88.

Research output: Contribution to journalArticle

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