Intracellular pH (pHi) in gastric surface epithelium is more susceptible to serosal than mucosal acidification

Stanley W. Ashley, David Soybel, C. Diane Moore, Laurence Y. Cheung

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Intracellular microelectrode techniques were used to examine the effects of mucosal or serosal acidification on intracellular pH (pHi) in gastric surface epithelial cells. Necturus antrum was mounted in a modified Ussing chamber, and pHi was determined from the difference between the potentials recorded by intracellular conventional and pH-sensitive microelectrodes. In tissues bathed with bicarbonate-buffered Ringer's solution (pH 7), acidification of the mucosal solution to pH 4.5 by isotonic replacement of the NaHCO3 with NaCl had no significant effects on pHi. In contrast, acidification of the serosal solution to pH 4.5 by replacing the bicarbonate reduced pHi from 7.32 ± 0.04 to 6.95 ± 0.06 (p < 0.001, n = 8). Similarly, in tissues bathed with HEPES-buffered Ringer's solution (pH 7.0), pHi was unaffected by reducing the mucosal solution pH to 4.5 with HCl but fell 0.21 ± 0.05 pH units (p < 0.01, n = 7) during acidification of the serosal solution to pH 6. These results suggest that gastric epithelium is more sensitive to acidification from the serosal than the mucosal side. Such a finding is consistent with the concept of a gastric mucosal barrier to luminal acid. It may also explain the gastric epithelium's greater sensitivity to acute ulceration during systemic acidosis.

Original languageEnglish (US)
Pages (from-to)371-379
Number of pages9
JournalSurgery
Volume102
Issue number2
StatePublished - Jan 1 1987

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Stomach
Epithelium
Microelectrodes
Bicarbonates
Necturus
HEPES
Acidosis
Epithelial Cells
Acids

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Ashley, Stanley W. ; Soybel, David ; Moore, C. Diane ; Cheung, Laurence Y. / Intracellular pH (pHi) in gastric surface epithelium is more susceptible to serosal than mucosal acidification. In: Surgery. 1987 ; Vol. 102, No. 2. pp. 371-379.
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Intracellular pH (pHi) in gastric surface epithelium is more susceptible to serosal than mucosal acidification. / Ashley, Stanley W.; Soybel, David; Moore, C. Diane; Cheung, Laurence Y.

In: Surgery, Vol. 102, No. 2, 01.01.1987, p. 371-379.

Research output: Contribution to journalArticle

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