TY - JOUR
T1 - Intracerebral microdialysis during deep brain stimulation surgery
AU - Kilpatrick, Michaux
AU - Church, Ephraim W.
AU - Danish, Shabbar
AU - Stiefel, Michael
AU - Jaggi, Jurg
AU - Halpern, Casey
AU - Kerr, Marie
AU - Maloney, Eileen
AU - Robinson, Michael
AU - Lucki, Irwin
AU - Krizman-Grenda, Elizabeth
AU - Baltuch, Gordon
PY - 2010/6
Y1 - 2010/6
N2 - Background: This report describes the use of microdialysis in conjunction with deep brain stimulation (DBS) surgery to assess extracellular levels of neurotransmitters within the human basal ganglia (BG). Electrical stimulation of the subthalamic nucleus (STN) is an efficacious treatment for advanced Parkinson's disease, yet the mechanisms of STN DBS remain poorly understood. Measurement of neurotransmitter levels within the BG may provide insight into mechanisms of DBS, but such an approach presents technical challenges.Methods: After microelectrode recordings confirmed location of STN, a custom microdialysis guide cannula was inserted. A CMA (Stockholm, Sweden) microdialysis probe was then positioned to the same depth as the microrecording electrode in STN or 2. mm inferiorly to record in the substantia nigra. The catheter was perfused at a rate of 2.0. μL/min with a sterile mock CSF solution and samples of extracellular fluid were collected at regular intervals. Dialysate samples were analyzed using high-pressure liquid chromatography (HPLC) detection procedures for quantitation of glutamate, gamma-aminobutyric acid (GABA), and dopamine.Results: Levels of neurotransmitters were reliably identified in dialysate samples using HPLC. By monitoring concentrations of glutamate, GABA and dopamine, we were able to demonstrate what seemed to be a steady state baseline within approximately 30. min.Conclusion: Microdialysis during DBS surgery is a feasible method for assessing levels of glutamate, GABA and dopamine within the human BG. Obtaining a steady state baseline of neurotransmitter levels appears feasible, thus making future studies of intraoperative microdialysis during DBS meaningful.
AB - Background: This report describes the use of microdialysis in conjunction with deep brain stimulation (DBS) surgery to assess extracellular levels of neurotransmitters within the human basal ganglia (BG). Electrical stimulation of the subthalamic nucleus (STN) is an efficacious treatment for advanced Parkinson's disease, yet the mechanisms of STN DBS remain poorly understood. Measurement of neurotransmitter levels within the BG may provide insight into mechanisms of DBS, but such an approach presents technical challenges.Methods: After microelectrode recordings confirmed location of STN, a custom microdialysis guide cannula was inserted. A CMA (Stockholm, Sweden) microdialysis probe was then positioned to the same depth as the microrecording electrode in STN or 2. mm inferiorly to record in the substantia nigra. The catheter was perfused at a rate of 2.0. μL/min with a sterile mock CSF solution and samples of extracellular fluid were collected at regular intervals. Dialysate samples were analyzed using high-pressure liquid chromatography (HPLC) detection procedures for quantitation of glutamate, gamma-aminobutyric acid (GABA), and dopamine.Results: Levels of neurotransmitters were reliably identified in dialysate samples using HPLC. By monitoring concentrations of glutamate, GABA and dopamine, we were able to demonstrate what seemed to be a steady state baseline within approximately 30. min.Conclusion: Microdialysis during DBS surgery is a feasible method for assessing levels of glutamate, GABA and dopamine within the human BG. Obtaining a steady state baseline of neurotransmitter levels appears feasible, thus making future studies of intraoperative microdialysis during DBS meaningful.
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U2 - 10.1016/j.jneumeth.2010.04.013
DO - 10.1016/j.jneumeth.2010.04.013
M3 - Article
C2 - 20416339
AN - SCOPUS:77953724948
SN - 0165-0270
VL - 190
SP - 106
EP - 111
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1
ER -
