Intramuscular injection of plasmid DNA encoding cottontail rabbit papillomavirus E1, E2, E6 and E7 induces T cell-mediated but not humoral immune responses in rabbits

Ricai Han, Cynthia A. Reed, Nancy M. Cladel, Neil Christensen

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

To test the efficacy of genetic vaccination against papillomavirus infection, plasmid DNA encoding cottontail rabbit papillomavirus (CRPV) E1, E2, E6, E7 or without insert were intramuscularly injected into five groups of rabbits. Peripheral blood mononuclear cells (PBMCs) showed specific proliferation upon in vitro stimulation with E1, E2, E6 or E7 proteins in a majority of vaccinated rabbits but Western blot analysis did not detect antibodies specific for these viral proteins in rabbit serum. All rabbits grew papillomas after virus challenge and none of the rabbits showed systemic papilloma regression. These observations showed that intramuscular injection of plasmid DNA encoding CRPV E1, E2, E6 or E7 induced CD4+ T cell-mediated but not humoral immune responses, and did not result in the protection of rabbits from virus infection.

Original languageEnglish (US)
Pages (from-to)1558-1566
Number of pages9
JournalVaccine
Volume17
Issue number11-12
DOIs
StatePublished - Mar 1 1999

Fingerprint

Cottontail rabbit papillomavirus
Intramuscular Injections
Humoral Immunity
intramuscular injection
humoral immunity
plasmids
Plasmids
T-lymphocytes
rabbits
Rabbits
T-Lymphocytes
DNA
papilloma
Papilloma
viruses
Papillomavirus Infections
Papillomaviridae
viral proteins
Viral Proteins
Virus Diseases

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

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title = "Intramuscular injection of plasmid DNA encoding cottontail rabbit papillomavirus E1, E2, E6 and E7 induces T cell-mediated but not humoral immune responses in rabbits",
abstract = "To test the efficacy of genetic vaccination against papillomavirus infection, plasmid DNA encoding cottontail rabbit papillomavirus (CRPV) E1, E2, E6, E7 or without insert were intramuscularly injected into five groups of rabbits. Peripheral blood mononuclear cells (PBMCs) showed specific proliferation upon in vitro stimulation with E1, E2, E6 or E7 proteins in a majority of vaccinated rabbits but Western blot analysis did not detect antibodies specific for these viral proteins in rabbit serum. All rabbits grew papillomas after virus challenge and none of the rabbits showed systemic papilloma regression. These observations showed that intramuscular injection of plasmid DNA encoding CRPV E1, E2, E6 or E7 induced CD4+ T cell-mediated but not humoral immune responses, and did not result in the protection of rabbits from virus infection.",
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Intramuscular injection of plasmid DNA encoding cottontail rabbit papillomavirus E1, E2, E6 and E7 induces T cell-mediated but not humoral immune responses in rabbits. / Han, Ricai; Reed, Cynthia A.; Cladel, Nancy M.; Christensen, Neil.

In: Vaccine, Vol. 17, No. 11-12, 01.03.1999, p. 1558-1566.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Christensen, Neil

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