Intrapreoptic microinjection of GHRH or its antagonist alters sleep in rats

Jianyi Zhang, Ferenc Obál, Tong Zheng, Jidong Fang, Ping Taishi, James M. Krueger

Research output: Contribution to journalArticle

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Abstract

Previous reports indicate that growth hormone-releasing hormone (GHRH) is involved in sleep regulation. The site of action mediating the nonrapid eye movement sleep (NREMS)-promoting effects of GHRH is not known, but it is independent from the pituitary. GHRH (0.001, 0.01, and 0.1 nmol/kg) or a competitive antagonist of GHRH (0.003, 0.3, and 14 nmol/kg) was microinjected into the preoptic area, and the sleep-wake activity was recorded for 23 hr after injection in rats. GHRH elicited dose-dependent increases in the duration and in the intensity of NREMS compared with that in control records after intrapreoptic injection of physiological saline. The antagonist decreased the duration and intensity of NREMS and prolonged sleep latency. Consistent alterations in rapid eye movement sleep (REMS) and in brain temperature were not found. The GHRH antagonist also attenuated the enhancements in NREMS elicited by 3 hr of sleep deprivation. Histological verification of the injection sites showed that the majority of the effective injections were in the preoptic area and the diagonal band of Broca. The results indicate that the preoptic area mediates the sleep-promoting activity of GHRH.

Original languageEnglish (US)
Pages (from-to)2187-2194
Number of pages8
JournalJournal of Neuroscience
Volume19
Issue number6
StatePublished - Mar 15 1999

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Growth Hormone-Releasing Hormone
Microinjections
Sleep
Eye Movements
Preoptic Area
Injections
Diagonal Band of Broca
Hormone Antagonists
Sleep Deprivation
REM Sleep
Temperature

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Zhang, J., Obál, F., Zheng, T., Fang, J., Taishi, P., & Krueger, J. M. (1999). Intrapreoptic microinjection of GHRH or its antagonist alters sleep in rats. Journal of Neuroscience, 19(6), 2187-2194.
Zhang, Jianyi ; Obál, Ferenc ; Zheng, Tong ; Fang, Jidong ; Taishi, Ping ; Krueger, James M. / Intrapreoptic microinjection of GHRH or its antagonist alters sleep in rats. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 6. pp. 2187-2194.
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Zhang, J, Obál, F, Zheng, T, Fang, J, Taishi, P & Krueger, JM 1999, 'Intrapreoptic microinjection of GHRH or its antagonist alters sleep in rats', Journal of Neuroscience, vol. 19, no. 6, pp. 2187-2194.

Intrapreoptic microinjection of GHRH or its antagonist alters sleep in rats. / Zhang, Jianyi; Obál, Ferenc; Zheng, Tong; Fang, Jidong; Taishi, Ping; Krueger, James M.

In: Journal of Neuroscience, Vol. 19, No. 6, 15.03.1999, p. 2187-2194.

Research output: Contribution to journalArticle

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Zhang J, Obál F, Zheng T, Fang J, Taishi P, Krueger JM. Intrapreoptic microinjection of GHRH or its antagonist alters sleep in rats. Journal of Neuroscience. 1999 Mar 15;19(6):2187-2194.