Intraspecific and interspecific variation in thermotolerance and photoacclimation in Symbiodinium dinoflagellates

Erika M. Díaz-Almeyda, C. Prada, A. H. Ohdera, H. Moran, D. J. Civitello, Roberto Iglesias-Prieto, Tomas A. Carlo-Joglar, Todd C. Lajeunesse, Monica Medina

Research output: Contribution to journalArticle

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Abstract

Light and temperature are major drivers in the ecology and biogeography of symbiotic dinoflagellates living in corals and other cnidarians. We examined variations in physiology among 11 strains comprising five species of clade A Symbiodinium. We grew cultures at 26oC (control) and 32oC (high temperature) over a duration of 18 days while measuring growth and photochemical efficiency (Fv/Fm). Responses to thermal stress ranged from susceptible to tolerant across species and strains. Most strains exhibited a decrease in cell densities and Fv/Fm when grown at 32oC. Tolerance to high temperature (T32) was calculated for all strains, ranging from 0 (unable to survive at high temperature) to 1 (able survive at high temperature). There was substantial variation in thermotolerance across species and among strains. One strain had a T32 close to 1, indicating that growth was not reduced at 32oC for only this one strain. To evaluate the combined effect of temperature and light on physiological stress, we selected three strains with different levels of thermotolerance (tolerant, intermediate and susceptible) and grew them under five different light intensities (65, 80, 100, 240 and 443 μmol quanta m-2 s-1) at 26 and 32oC. High irradiance exacerbated the effect of high temperature, particularly in strains from thermally sensitive species. This work further supports the recognition that broad physiological differences exist not only among species within Symbiodinium clades, but also among strains within species demonstrating that thermotolerance varies widely between species and among strains within species.

Original languageEnglish (US)
Article number20171767
JournalProceedings of the Royal Society B: Biological Sciences
Volume284
Issue number1868
DOIs
StatePublished - Dec 6 2017

Fingerprint

Dinoflagellida
Symbiodinium
interspecific variation
intraspecific variation
heat tolerance
dinoflagellate
Temperature
Light
temperature
Cnidaria
Anthozoa
Physiological Stress
Physiology
Ecology
Growth
Miozoa
Thermotolerance
Thermal stress
biogeography
light intensity

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "Intraspecific and interspecific variation in thermotolerance and photoacclimation in Symbiodinium dinoflagellates",
abstract = "Light and temperature are major drivers in the ecology and biogeography of symbiotic dinoflagellates living in corals and other cnidarians. We examined variations in physiology among 11 strains comprising five species of clade A Symbiodinium. We grew cultures at 26oC (control) and 32oC (high temperature) over a duration of 18 days while measuring growth and photochemical efficiency (Fv/Fm). Responses to thermal stress ranged from susceptible to tolerant across species and strains. Most strains exhibited a decrease in cell densities and Fv/Fm when grown at 32oC. Tolerance to high temperature (T32) was calculated for all strains, ranging from 0 (unable to survive at high temperature) to 1 (able survive at high temperature). There was substantial variation in thermotolerance across species and among strains. One strain had a T32 close to 1, indicating that growth was not reduced at 32oC for only this one strain. To evaluate the combined effect of temperature and light on physiological stress, we selected three strains with different levels of thermotolerance (tolerant, intermediate and susceptible) and grew them under five different light intensities (65, 80, 100, 240 and 443 μmol quanta m-2 s-1) at 26 and 32oC. High irradiance exacerbated the effect of high temperature, particularly in strains from thermally sensitive species. This work further supports the recognition that broad physiological differences exist not only among species within Symbiodinium clades, but also among strains within species demonstrating that thermotolerance varies widely between species and among strains within species.",
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Intraspecific and interspecific variation in thermotolerance and photoacclimation in Symbiodinium dinoflagellates. / Díaz-Almeyda, Erika M.; Prada, C.; Ohdera, A. H.; Moran, H.; Civitello, D. J.; Iglesias-Prieto, Roberto; Carlo-Joglar, Tomas A.; Lajeunesse, Todd C.; Medina, Monica.

In: Proceedings of the Royal Society B: Biological Sciences, Vol. 284, No. 1868, 20171767, 06.12.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Intraspecific and interspecific variation in thermotolerance and photoacclimation in Symbiodinium dinoflagellates

AU - Díaz-Almeyda, Erika M.

AU - Prada, C.

AU - Ohdera, A. H.

AU - Moran, H.

AU - Civitello, D. J.

AU - Iglesias-Prieto, Roberto

AU - Carlo-Joglar, Tomas A.

AU - Lajeunesse, Todd C.

AU - Medina, Monica

PY - 2017/12/6

Y1 - 2017/12/6

N2 - Light and temperature are major drivers in the ecology and biogeography of symbiotic dinoflagellates living in corals and other cnidarians. We examined variations in physiology among 11 strains comprising five species of clade A Symbiodinium. We grew cultures at 26oC (control) and 32oC (high temperature) over a duration of 18 days while measuring growth and photochemical efficiency (Fv/Fm). Responses to thermal stress ranged from susceptible to tolerant across species and strains. Most strains exhibited a decrease in cell densities and Fv/Fm when grown at 32oC. Tolerance to high temperature (T32) was calculated for all strains, ranging from 0 (unable to survive at high temperature) to 1 (able survive at high temperature). There was substantial variation in thermotolerance across species and among strains. One strain had a T32 close to 1, indicating that growth was not reduced at 32oC for only this one strain. To evaluate the combined effect of temperature and light on physiological stress, we selected three strains with different levels of thermotolerance (tolerant, intermediate and susceptible) and grew them under five different light intensities (65, 80, 100, 240 and 443 μmol quanta m-2 s-1) at 26 and 32oC. High irradiance exacerbated the effect of high temperature, particularly in strains from thermally sensitive species. This work further supports the recognition that broad physiological differences exist not only among species within Symbiodinium clades, but also among strains within species demonstrating that thermotolerance varies widely between species and among strains within species.

AB - Light and temperature are major drivers in the ecology and biogeography of symbiotic dinoflagellates living in corals and other cnidarians. We examined variations in physiology among 11 strains comprising five species of clade A Symbiodinium. We grew cultures at 26oC (control) and 32oC (high temperature) over a duration of 18 days while measuring growth and photochemical efficiency (Fv/Fm). Responses to thermal stress ranged from susceptible to tolerant across species and strains. Most strains exhibited a decrease in cell densities and Fv/Fm when grown at 32oC. Tolerance to high temperature (T32) was calculated for all strains, ranging from 0 (unable to survive at high temperature) to 1 (able survive at high temperature). There was substantial variation in thermotolerance across species and among strains. One strain had a T32 close to 1, indicating that growth was not reduced at 32oC for only this one strain. To evaluate the combined effect of temperature and light on physiological stress, we selected three strains with different levels of thermotolerance (tolerant, intermediate and susceptible) and grew them under five different light intensities (65, 80, 100, 240 and 443 μmol quanta m-2 s-1) at 26 and 32oC. High irradiance exacerbated the effect of high temperature, particularly in strains from thermally sensitive species. This work further supports the recognition that broad physiological differences exist not only among species within Symbiodinium clades, but also among strains within species demonstrating that thermotolerance varies widely between species and among strains within species.

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