Intraspecific competition impacts Vibrio fischeri strain diversity during initial colonization of the squid light organ

Yan Sun, Elijah D. LaSota, Andrew G. Cecere, Kyle B. LaPenna, Jessie Larios-Valencia, Michael S. Wollenberg, Timothy Iwao Miyashiro

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Animal development and physiology depend on beneficial interactions with microbial symbionts. In many cases, the microbial symbionts are horizontally transmitted among hosts, thereby making the acquisition of these microbes from the environment an important event within the life history of each host. The light organ symbiosis established between the Hawaiian squid Euprymna scolopes and the bioluminescent bacterium Vibrio fischeri is a model system for examining how hosts acquire horizontally transmitted microbial symbionts. Recent studies have revealed that the light organ of wild-caught E. scolopes squid contains polyclonal populations of V. fischeri bacteria; however, the function and development of such strain diversity in the symbiosis are unknown. Here, we report our phenotypic and phylogenetic characterizations of FQ-A001, which is a V. fischeri strain isolated directly from the light organ of an E. scolopes individual. Relative to the type strain ES114, FQ-A001 exhibits similar growth in rich medium but displays increased bioluminescence and decreased motility in soft agar. FQ-A001 outcompetes ES114 in colonizing the crypt spaces of the light organs. Remarkably, we find that animals cocolonized with FQ-A001 and ES114 harbor singly colonized crypts, in contrast to the cocolonized crypts observed from competition experiments involving single genotypes. The results with our two-strain system suggest that strain diversity within the squid light organ is a consequence of diversity in the single-strain colonization of individual crypt spaces.

Original languageEnglish (US)
Pages (from-to)3082-3091
Number of pages10
JournalApplied and environmental microbiology
Volume82
Issue number10
DOIs
StatePublished - May 1 2016

Fingerprint

Aliivibrio fischeri
Vibrio fischeri
Decapodiformes
strain differences
intraspecific competition
squid
colonization
Light
symbiont
symbionts
Symbiosis
symbiosis
Microbial Interactions
animal physiology
Bacteria
animal development
bioluminescence
bacterium
animal
bacteria

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Sun, Yan ; LaSota, Elijah D. ; Cecere, Andrew G. ; LaPenna, Kyle B. ; Larios-Valencia, Jessie ; Wollenberg, Michael S. ; Miyashiro, Timothy Iwao. / Intraspecific competition impacts Vibrio fischeri strain diversity during initial colonization of the squid light organ. In: Applied and environmental microbiology. 2016 ; Vol. 82, No. 10. pp. 3082-3091.
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Intraspecific competition impacts Vibrio fischeri strain diversity during initial colonization of the squid light organ. / Sun, Yan; LaSota, Elijah D.; Cecere, Andrew G.; LaPenna, Kyle B.; Larios-Valencia, Jessie; Wollenberg, Michael S.; Miyashiro, Timothy Iwao.

In: Applied and environmental microbiology, Vol. 82, No. 10, 01.05.2016, p. 3082-3091.

Research output: Contribution to journalArticle

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