@article{6395dd894ab5493d84a0110501f4bc30,
title = "Introduced bees (Osmia cornifrons) collect pollen from both coevolved and novel host-plant species within their family-level phylogenetic preferences",
abstract = "Studying the pollen preferences of introduced bees allows us to investigate how species use host-plants when establishing in new environments. Osmia cornifrons is a solitary bee introduced into North America from East Asia for pollination of Rosaceae crops such as apples and cherries. We investigated whether O. cornifrons (i) more frequently collected pollen from host-plant species they coevolved with from their geographic origin, or (ii) prefer host-plant species of specific plant taxa independent of origin. To address this question, using pollen metabarcoding, we examined the identity and relative abundance of pollen in larval provisions from nests located in different landscapes with varying abundance of East-Asian and non-Asian plant species. Our results show that O. cornifrons collected more pollen from plant species from their native range. Plants in the family Rosaceae were their most preferred pollen hosts, but they differentially collected species native to East Asia, Europe, or North America depending on the landscape. Our results suggest that while O. cornifrons frequently collect pollen of East-Asian origin, the collection of pollen from novel species within their phylogenetic familial affinities is common and can facilitate pollinator establishment. This phylogenetic preference highlights the effectiveness of O. cornifrons as crop pollinators of a variety of Rosaceae crops from different geographic origins. Our results imply that globalization of non-native plant species may ease the naturalization of their coevolved pollinators outside of their native range.",
author = "Vaudo, {Anthony D.} and Biddinger, {David J.} and Wiebke Sickel and Alexander Keller and L{\'o}pez-Uribe, {Margarita M.}",
note = "Funding Information: Data accessibility. Available in Dryad: (i) pollen barcoded sequences, (ii) sample metadata, (iii) ITS2 reference database of plants of Pennsylvania, (iv) code for quality filtering, sequence alignment, and determining taxonomic and OTU tables, (v) resulting combined taxonomic and abundance table, (vi) code for taxa filtering and diversity indices, and (vii) final dataset used for analysis. Supporting figures are available in electronic supplementary material. https://doi.org/10.5061/dryad.ffbg79cqn [59]. Authors{\textquoteright} contributions. A.D.V., D.J.B. and M.M.L.-U. designed the study. A.D.V. conducted molecular lab work and data analysis. D.J.B. collected pollen samples. W.S. assisted in laboratory techniques. A.K. conducted sequence quality control and classification. A.D.V. and M.M.L.-U. drafted the manuscript. All authors critically revised the manuscript and gave final approval for publication. Competing interests. We declare we have no competing interests. Funding. This work was supported by the USDA-SCRI grant PEN04398, USDA-SCRI Coordinated Agricultural Project grant no. MICL05063, USDA Hatch Appropriations under Project PEN04619, and the State Horticultural Association of Pennsylvania. Permission to carry out fieldwork. No permissions were required prior to conducting field work; all sites are privately owned and work in coordination with Penn State Fruit Research and Extension Center. Publisher Copyright: {\textcopyright} 2020 The Authors.",
year = "2020",
month = jul,
day = "1",
doi = "10.1098/rsos.200225",
language = "English (US)",
volume = "7",
journal = "Royal Society Open Science",
issn = "2054-5703",
publisher = "The Royal Society",
number = "7",
}