Investigation of the reliability of 24 h urine excretion as a biomarker of isoflavone exposure over time and over a wide range of isoflavone intakes

M. R. Ritchie, M. S. Morton, A. M. Thompson, Nigel Deighton, A. Blake, J. H. Cummings, C. M. Steel

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective: To study the variation in genistein + daidzein intake over a 6-month period and test the reliability of 24 h urinary isoflavones as a biomarker of exposure over time. Design: Dietary genistein + daidzein intake was assessed at various time points throughout six months in 15 healthy subjects. Group 1 (n = 8) followed nonsupplemented diets and Group 2 (n = 7) took a 35 mg/d isoflavone supplement for 3 months and each subject provided a 24 h urine collection, validated with para-aminobenzoic acid, during weeks 7, 15 and 19. Urine was analysed for genistein and daidzein using LC-MS. Results: Isoflavone intake in Groups 1 and 2 ranged from 0.00 to 1.1 mg/d and 0.1 to 53.1 mg/d, respectively. Urine excretion for both groups ranged from 0.20 to 9.56 mg/d. The relationship between 24h excretion and isoflavone intake is y= 0.44 × ± 0.03(standard deviation) + 1.57; r= 0.89, P< 0.001. Conclusion: The 24 h urinary isoflavones can be used as biomarkers of isoflavone exposure over time.

Original languageEnglish (US)
Pages (from-to)1286-1289
Number of pages4
JournalEuropean Journal of Clinical Nutrition
Volume58
Issue number9
DOIs
StatePublished - Sep 1 2004

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Isoflavones
Biomarkers
Urine
Genistein
4-Aminobenzoic Acid
Urine Specimen Collection
Healthy Volunteers
Diet
daidzein

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Ritchie, M. R. ; Morton, M. S. ; Thompson, A. M. ; Deighton, Nigel ; Blake, A. ; Cummings, J. H. ; Steel, C. M. / Investigation of the reliability of 24 h urine excretion as a biomarker of isoflavone exposure over time and over a wide range of isoflavone intakes. In: European Journal of Clinical Nutrition. 2004 ; Vol. 58, No. 9. pp. 1286-1289.
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Investigation of the reliability of 24 h urine excretion as a biomarker of isoflavone exposure over time and over a wide range of isoflavone intakes. / Ritchie, M. R.; Morton, M. S.; Thompson, A. M.; Deighton, Nigel; Blake, A.; Cummings, J. H.; Steel, C. M.

In: European Journal of Clinical Nutrition, Vol. 58, No. 9, 01.09.2004, p. 1286-1289.

Research output: Contribution to journalArticle

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T1 - Investigation of the reliability of 24 h urine excretion as a biomarker of isoflavone exposure over time and over a wide range of isoflavone intakes

AU - Ritchie, M. R.

AU - Morton, M. S.

AU - Thompson, A. M.

AU - Deighton, Nigel

AU - Blake, A.

AU - Cummings, J. H.

AU - Steel, C. M.

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N2 - Objective: To study the variation in genistein + daidzein intake over a 6-month period and test the reliability of 24 h urinary isoflavones as a biomarker of exposure over time. Design: Dietary genistein + daidzein intake was assessed at various time points throughout six months in 15 healthy subjects. Group 1 (n = 8) followed nonsupplemented diets and Group 2 (n = 7) took a 35 mg/d isoflavone supplement for 3 months and each subject provided a 24 h urine collection, validated with para-aminobenzoic acid, during weeks 7, 15 and 19. Urine was analysed for genistein and daidzein using LC-MS. Results: Isoflavone intake in Groups 1 and 2 ranged from 0.00 to 1.1 mg/d and 0.1 to 53.1 mg/d, respectively. Urine excretion for both groups ranged from 0.20 to 9.56 mg/d. The relationship between 24h excretion and isoflavone intake is y= 0.44 × ± 0.03(standard deviation) + 1.57; r= 0.89, P< 0.001. Conclusion: The 24 h urinary isoflavones can be used as biomarkers of isoflavone exposure over time.

AB - Objective: To study the variation in genistein + daidzein intake over a 6-month period and test the reliability of 24 h urinary isoflavones as a biomarker of exposure over time. Design: Dietary genistein + daidzein intake was assessed at various time points throughout six months in 15 healthy subjects. Group 1 (n = 8) followed nonsupplemented diets and Group 2 (n = 7) took a 35 mg/d isoflavone supplement for 3 months and each subject provided a 24 h urine collection, validated with para-aminobenzoic acid, during weeks 7, 15 and 19. Urine was analysed for genistein and daidzein using LC-MS. Results: Isoflavone intake in Groups 1 and 2 ranged from 0.00 to 1.1 mg/d and 0.1 to 53.1 mg/d, respectively. Urine excretion for both groups ranged from 0.20 to 9.56 mg/d. The relationship between 24h excretion and isoflavone intake is y= 0.44 × ± 0.03(standard deviation) + 1.57; r= 0.89, P< 0.001. Conclusion: The 24 h urinary isoflavones can be used as biomarkers of isoflavone exposure over time.

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