Investigation of the specificity of O6-alkylguanine-DNA-alkyltransferase

Anthony E. Pegg; Kazushige Morimoto; M. Eileen Dolan

doi:10.1016/0009-2797(88)90112-3

Investigation of the specificity of O⁶-alkylguanine-DNA-alkyltransferase

Anthony E. Pegg, Kazushige Morimoto, M. Eileen Dolan

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14 Scopus citations

Abstract

Comparison of the abilities of alkylated RNA and DNA to serve as substrates for the O⁶-alkylguanine-DNA-alkyltransferase have been carried out. It was found that the O⁶-methylguanine in tRNA was much less active as a substrate for the protein than O⁶-methylguanine in double stranded DNA. The difference in rates of repair was such that it is unlikely that the alkyltransferase would act on RNA in vivo and, therefore, the reaction with RNA should not contribute towards the exhaustion of its repair capacity.

Original language	English (US)
Pages (from-to)	275-281
Number of pages	7
Journal	Chemico-Biological Interactions
Volume	65
Issue number	3
DOIs	https://doi.org/10.1016/0009-2797(88)90112-3
State	Published - 1988

All Science Journal Classification (ASJC) codes

Toxicology

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10.1016/0009-2797(88)90112-3

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title = "Investigation of the specificity of O6-alkylguanine-DNA-alkyltransferase",

abstract = "Comparison of the abilities of alkylated RNA and DNA to serve as substrates for the O6-alkylguanine-DNA-alkyltransferase have been carried out. It was found that the O6-methylguanine in tRNA was much less active as a substrate for the protein than O6-methylguanine in double stranded DNA. The difference in rates of repair was such that it is unlikely that the alkyltransferase would act on RNA in vivo and, therefore, the reaction with RNA should not contribute towards the exhaustion of its repair capacity.",

author = "Pegg, {Anthony E.} and Kazushige Morimoto and Dolan, {M. Eileen}",

note = "Funding Information: This research was supported by NIH grant CA-18137.",

year = "1988",

doi = "10.1016/0009-2797(88)90112-3",

language = "English (US)",

volume = "65",

pages = "275--281",

journal = "Chemico-Biological Interactions",

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T1 - Investigation of the specificity of O6-alkylguanine-DNA-alkyltransferase

AU - Pegg, Anthony E.

AU - Morimoto, Kazushige

AU - Dolan, M. Eileen

N1 - Funding Information: This research was supported by NIH grant CA-18137.

PY - 1988

Y1 - 1988

N2 - Comparison of the abilities of alkylated RNA and DNA to serve as substrates for the O6-alkylguanine-DNA-alkyltransferase have been carried out. It was found that the O6-methylguanine in tRNA was much less active as a substrate for the protein than O6-methylguanine in double stranded DNA. The difference in rates of repair was such that it is unlikely that the alkyltransferase would act on RNA in vivo and, therefore, the reaction with RNA should not contribute towards the exhaustion of its repair capacity.

AB - Comparison of the abilities of alkylated RNA and DNA to serve as substrates for the O6-alkylguanine-DNA-alkyltransferase have been carried out. It was found that the O6-methylguanine in tRNA was much less active as a substrate for the protein than O6-methylguanine in double stranded DNA. The difference in rates of repair was such that it is unlikely that the alkyltransferase would act on RNA in vivo and, therefore, the reaction with RNA should not contribute towards the exhaustion of its repair capacity.

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U2 - 10.1016/0009-2797(88)90112-3

DO - 10.1016/0009-2797(88)90112-3

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EP - 281

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

IS - 3

ER -

Investigation of the specificity of O⁶-alkylguanine-DNA-alkyltransferase

Abstract

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