Investigation of the specificity of O6-alkylguanine-DNA-alkyltransferase

Anthony E. Pegg, Kazushige Morimoto, M. Eileen Dolan

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Comparison of the abilities of alkylated RNA and DNA to serve as substrates for the O6-alkylguanine-DNA-alkyltransferase have been carried out. It was found that the O6-methylguanine in tRNA was much less active as a substrate for the protein than O6-methylguanine in double stranded DNA. The difference in rates of repair was such that it is unlikely that the alkyltransferase would act on RNA in vivo and, therefore, the reaction with RNA should not contribute towards the exhaustion of its repair capacity.

Original languageEnglish (US)
Pages (from-to)275-281
Number of pages7
JournalChemico-Biological Interactions
Volume65
Issue number3
DOIs
StatePublished - 1988

All Science Journal Classification (ASJC) codes

  • Toxicology

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