基于转录组和蛋白互作网络分析光信号在花药发育过程中的功能和可能机制

Translated title of the contribution: Investigation on the function and possible mechanism of light signal in anther development based on transcriptome and protein interaction network analyses

Lin Wei Ruan, Ying Fu, Meng Han Wang, Meng Yu Li, Hong Ma, Fang Chang

Research output: Contribution to journalArticle

Abstract

Anther is the male reproductive organ of angiosperms, and its normal development is crucial to plant sexual reproduction and crop yield. Anther development is regulated by both intrinsic and external environmental signals. Light is one of the most important environmental factors and affects almost all aspects of the plant growth and development. However, there are few reports on whether light participates and how to participate in the regulation of anther development. Here in this study, 752 (96.66%) of light-related genes were found to be expressed in stages 4-7 anthers of Arabidopsis thaliana, of which 246 were highly expressed and 33 were extremely highly expressed; 655 were expressed in stages 4-7 tapetum and 633 in stages 8-10 tapetum, suggesting that these genes might play important roles in anther development. The expression of light-related genes in dyt1-3, bhlh010 bhlh089 bhlh091 and ams mutant anthers was further analyzed, and a number of light-related genes regulated by these transcription factors were identified, suggesting that these genes play a role as downstream components of the DYT1-bHLHs-AMS transcriptional network. In addition, proteins related to the photo systems and light signal transduction pathway that interact with bHLH010, bHLH089, bHLH091 and DYT1 were screened with yeast two-hybrid experiment, and the interaction network of these proteins was drawn. This study provides a new understanding and important reference for further revealing the function of light signaling genes and their regulatory mechanism in anther development.

Original languageChinese
Pages (from-to)159-168
Number of pages10
JournalZhiwu Shengli Xuebao/Plant Physiology Journal
Volume55
Issue number2
DOIs
StatePublished - Feb 20 2019

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Protein Interaction Maps
Transcriptome
transcriptome
anthers
Light
proteins
Genes
genes
Light Signal Transduction
Angiosperms
Plant Development
phototransduction
Gene Regulatory Networks
plant reproduction
Regulator Genes
Growth and Development
Arabidopsis
Reproduction
sexual reproduction
Signal Transduction

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Ruan, Lin Wei ; Fu, Ying ; Wang, Meng Han ; Li, Meng Yu ; Ma, Hong ; Chang, Fang. / 基于转录组和蛋白互作网络分析光信号在花药发育过程中的功能和可能机制. In: Zhiwu Shengli Xuebao/Plant Physiology Journal. 2019 ; Vol. 55, No. 2. pp. 159-168.
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基于转录组和蛋白互作网络分析光信号在花药发育过程中的功能和可能机制. / Ruan, Lin Wei; Fu, Ying; Wang, Meng Han; Li, Meng Yu; Ma, Hong; Chang, Fang.

In: Zhiwu Shengli Xuebao/Plant Physiology Journal, Vol. 55, No. 2, 20.02.2019, p. 159-168.

Research output: Contribution to journalArticle

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