Investigations into the role of the plastidial peptide methionine sulfoxide reductase in response to oxidative stress in Arabidopsis.

Hernán M. Romero, Barbara S. Berlett, Philip J. Jensen, Eva J. Pell, Ming Tien

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Peptidyl Met residues are readily oxidized by reactive oxygen species to form Met sulfoxide. The enzyme peptide Met sulfoxide reductase (PMSR) catalyzes the reduction of Met sulfoxides back to Met. In doing so, PMSR is proposed to act as a last-chance antioxidant, repairing proteins damaged from oxidative stress. To assess the role of this enzyme in plants, we generated multiple transgenic lines with altered expression levels of the plastid form of PMSR (PMSR4). In transgenic plants, PMSR4 expression ranged from 95% to 40% (antisense) and more than 600% (overexpressing lines) of wild-type plants. Under optimal growing conditions, there is no effect of the transgene on the phenotype of the plants. When exposed to different oxidative stress conditions-methyl viologen, ozone, and high light-differences were observed in the rate of photosynthesis, the maximum quantum yield (Fv/Fm ratio), and the Met sulfoxide content of the isolated chloroplast. Plants that overexpressed PMSR4 were more resistant to oxidative damage localized in the chloroplast, and plants that underexpressed PMSR4 were more susceptible. The Met sulfoxide levels in proteins of the soluble fraction of chloroplasts were increased by methyl viologen and ozone, but not by high-light treatment. Under stress conditions, the overexpression of PMSR4 lowered the sulfoxide content and underexpression resulted in an overall increase in content.

Original languageEnglish (US)
Pages (from-to)3784-3794
Number of pages11
JournalPlant physiology
Volume136
Issue number3
DOIs
StatePublished - Nov 2004

Fingerprint

sulfoxide
Arabidopsis
methionine
Oxidative Stress
oxidative stress
peptides
Chloroplasts
Paraquat
chloroplasts
paraquat
Ozone
ozone
Peptides
Sulfoxides
Light
Plastids
Genetically Modified Plants
Photosynthesis
Enzymes
enzymes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Romero, Hernán M. ; Berlett, Barbara S. ; Jensen, Philip J. ; Pell, Eva J. ; Tien, Ming. / Investigations into the role of the plastidial peptide methionine sulfoxide reductase in response to oxidative stress in Arabidopsis. In: Plant physiology. 2004 ; Vol. 136, No. 3. pp. 3784-3794.
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Investigations into the role of the plastidial peptide methionine sulfoxide reductase in response to oxidative stress in Arabidopsis. / Romero, Hernán M.; Berlett, Barbara S.; Jensen, Philip J.; Pell, Eva J.; Tien, Ming.

In: Plant physiology, Vol. 136, No. 3, 11.2004, p. 3784-3794.

Research output: Contribution to journalArticle

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