Involvement of rppH in thermoregulation in Pseudomonas syringae

Kevin Hockett, Michael Ionescu, Steven E. Lindow

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Temperature, among other environmental factors, influences the incidence and severity of many plant diseases. Likewise, numerous traits, including the expression of virulence factors, are regulated by temperature. Little is known about the underlying genetic determinants of thermoregulation in plant-pathogenic bacteria. Previously, we showed that the expression of both fliC (encoding flagellin) and syfA (encoding a nonribosomal polypeptide synthetase) was suppressed at high temperatures in Pseudomonas syringae. In this work, we used a high-throughput screen to identify mutations that conferred overexpression of syfA at elevated temperatures (28°C compared to 20°C). Two genes, Psyr_2474, encoding an acyl-coenzyme A (CoA) dehydrogenase, and Psyr_4843, encoding an ortholog of RppH, which in Escherichia coli mediates RNA turnover, contribute to thermoregulation of syfA. To assess the global role of rppH in thermoregulation in P. syringae, RNA sequencing was used to compare the transcriptomes of an rppH deletion mutant and the wild-type strain incubated at 20°C and 30°C. The disruption of rppH had a large effect on the temperature-dependent transcriptome of P. syringae, affecting the expression of 569 genes at either 20°C or 30°C but not at both temperatures. Intriguingly, RppH is involved in the thermoregulation of ribosome-associated proteins, as well as of RNase E, suggesting a prominent role of rppH on the proteome in addition to its effect on the transcriptome.

Original languageEnglish (US)
Pages (from-to)2313-2322
Number of pages10
JournalJournal of bacteriology
Volume196
Issue number12
DOIs
StatePublished - Jan 1 2014

Fingerprint

Pseudomonas syringae
Body Temperature Regulation
Temperature
Transcriptome
Acyl-CoA Dehydrogenase
RNA Sequence Analysis
Plant Diseases
Flagellin
Virulence Factors
Proteome
Ligases
Ribosomes
RNA
Escherichia coli
Bacteria
Gene Expression
Peptides
Mutation
Incidence
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Hockett, Kevin ; Ionescu, Michael ; Lindow, Steven E. / Involvement of rppH in thermoregulation in Pseudomonas syringae. In: Journal of bacteriology. 2014 ; Vol. 196, No. 12. pp. 2313-2322.
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Involvement of rppH in thermoregulation in Pseudomonas syringae. / Hockett, Kevin; Ionescu, Michael; Lindow, Steven E.

In: Journal of bacteriology, Vol. 196, No. 12, 01.01.2014, p. 2313-2322.

Research output: Contribution to journalArticle

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