Iodination of plasma membrane proteins of BHK cells in different growth states

Andrea Marie Mastro, Charles T. Beer, Gerald C. Mueller

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The surfaces of BHK cells in confluent monolayers, immediately after mechanical dispersal and in logarithmically growing suspension cultures have been iodinated with 125I using the lactoperoxidase technique. Electrophoretic resolution of the labeled proteins revealed that the representation of plasma membrane proteins varies with the growth state. Trypsinization of the cells produced a drastic revision of the surfaces leaving behind root fragments of membrane components and exposing additional proteins for iodination. The rapid turnover of membrane proteins in growing BHK cells restored the plasma membrane to a state characteristic of the replicating cell within 10 h.

Original languageEnglish (US)
Pages (from-to)38-51
Number of pages14
JournalBBA - Biomembranes
Volume352
Issue number1
DOIs
StatePublished - May 30 1974

Fingerprint

Halogenation
Cell membranes
Blood Proteins
Membrane Proteins
Cell Membrane
Lactoperoxidase
Growth
Monolayers
Suspensions
Proteins
Membranes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Mastro, Andrea Marie ; Beer, Charles T. ; Mueller, Gerald C. / Iodination of plasma membrane proteins of BHK cells in different growth states. In: BBA - Biomembranes. 1974 ; Vol. 352, No. 1. pp. 38-51.
@article{ff502ade32da47cea93e49401a4e6cb0,
title = "Iodination of plasma membrane proteins of BHK cells in different growth states",
abstract = "The surfaces of BHK cells in confluent monolayers, immediately after mechanical dispersal and in logarithmically growing suspension cultures have been iodinated with 125I using the lactoperoxidase technique. Electrophoretic resolution of the labeled proteins revealed that the representation of plasma membrane proteins varies with the growth state. Trypsinization of the cells produced a drastic revision of the surfaces leaving behind root fragments of membrane components and exposing additional proteins for iodination. The rapid turnover of membrane proteins in growing BHK cells restored the plasma membrane to a state characteristic of the replicating cell within 10 h.",
author = "Mastro, {Andrea Marie} and Beer, {Charles T.} and Mueller, {Gerald C.}",
year = "1974",
month = "5",
day = "30",
doi = "10.1016/0005-2736(74)90177-1",
language = "English (US)",
volume = "352",
pages = "38--51",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "1",

}

Iodination of plasma membrane proteins of BHK cells in different growth states. / Mastro, Andrea Marie; Beer, Charles T.; Mueller, Gerald C.

In: BBA - Biomembranes, Vol. 352, No. 1, 30.05.1974, p. 38-51.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Iodination of plasma membrane proteins of BHK cells in different growth states

AU - Mastro, Andrea Marie

AU - Beer, Charles T.

AU - Mueller, Gerald C.

PY - 1974/5/30

Y1 - 1974/5/30

N2 - The surfaces of BHK cells in confluent monolayers, immediately after mechanical dispersal and in logarithmically growing suspension cultures have been iodinated with 125I using the lactoperoxidase technique. Electrophoretic resolution of the labeled proteins revealed that the representation of plasma membrane proteins varies with the growth state. Trypsinization of the cells produced a drastic revision of the surfaces leaving behind root fragments of membrane components and exposing additional proteins for iodination. The rapid turnover of membrane proteins in growing BHK cells restored the plasma membrane to a state characteristic of the replicating cell within 10 h.

AB - The surfaces of BHK cells in confluent monolayers, immediately after mechanical dispersal and in logarithmically growing suspension cultures have been iodinated with 125I using the lactoperoxidase technique. Electrophoretic resolution of the labeled proteins revealed that the representation of plasma membrane proteins varies with the growth state. Trypsinization of the cells produced a drastic revision of the surfaces leaving behind root fragments of membrane components and exposing additional proteins for iodination. The rapid turnover of membrane proteins in growing BHK cells restored the plasma membrane to a state characteristic of the replicating cell within 10 h.

UR - http://www.scopus.com/inward/record.url?scp=0016202693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016202693&partnerID=8YFLogxK

U2 - 10.1016/0005-2736(74)90177-1

DO - 10.1016/0005-2736(74)90177-1

M3 - Article

C2 - 4859371

AN - SCOPUS:0016202693

VL - 352

SP - 38

EP - 51

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

SN - 0005-2736

IS - 1

ER -