Iodination of plasma membrane proteins of BHK cells in different growth states

Andrea M. Mastro, Charles T. Beer, Gerald C. Mueller

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Abstract

The surfaces of BHK cells in confluent monolayers, immediately after mechanical dispersal and in logarithmically growing suspension cultures have been iodinated with 125I using the lactoperoxidase technique. Electrophoretic resolution of the labeled proteins revealed that the representation of plasma membrane proteins varies with the growth state. Trypsinization of the cells produced a drastic revision of the surfaces leaving behind root fragments of membrane components and exposing additional proteins for iodination. The rapid turnover of membrane proteins in growing BHK cells restored the plasma membrane to a state characteristic of the replicating cell within 10 h.

Original languageEnglish (US)
Pages (from-to)38-51
Number of pages14
JournalBBA - Biomembranes
Volume352
Issue number1
DOIs
StatePublished - May 30 1974

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

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