Ion-pulling simulations provide insights into the mechanisms of channel opening of the skeletal muscle ryanodine receptor

David D. Mowrey, Le Xu, Yingwu Mei, Daniel A. Pasek, Gerhard Meissner, Nikolay Dokholyan

Research output: Contribution to journalArticle

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Abstract

The type 1 ryanodine receptor (RyR1) mediates Ca2 release from the sarcoplasmic reticulum to initiate skeletal muscle contraction and is associated with muscle diseases, malignant hyperthermia, and central core disease. To better understand RyR1 channel function, we investigated the molecular mechanisms of channel gating and ion permeation. An adequate model of channel gating requires accurate, high-resolution models of both open and closed states of the channel. To this end, we generated an open-channel RyR1 model using molecular simulations to pull Ca2 through the pore constriction site of a closed-channel RyR1 structure determined at 3.8-Å resolution. Importantly, we find that our open-channel model is consistent with the RyR1 and cardiac RyR (RyR2) open-channel structures reported while this paper was in preparation. Both our model and the published structures show similar rotation of the upper portion of the pore-lining S6 helix away from the 4-fold channel axis and twisting of Ile-4937 at the channel constriction site out of the channel pore. These motions result in a minimum open-channel pore radius of 3 Å formed by Gln-4933, rather than Ile-4937 in the closed-channel structure. We also present functional support for our model by mutations around the closed- and open-channel constriction sites (Gln-4933 and Ile-4937). Our results indicate that use of ion-pulling simulations produces a RyR1 open-channel model, which can provide insights into the mechanisms of channel opening complementing those from the structural data.

Original languageEnglish (US)
Pages (from-to)12947-12958
Number of pages12
JournalJournal of Biological Chemistry
Volume292
Issue number31
DOIs
StatePublished - Jan 1 2017

Fingerprint

Ryanodine Receptor Calcium Release Channel
Muscle
Skeletal Muscle
Ions
Constriction
Central Core Myopathy
Ion Channel Gating
S 6
Malignant Hyperthermia
Molecular Models
Sarcoplasmic Reticulum
Muscle Contraction
Ion Channels
Linings
Permeation
Muscles
Mutation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Mowrey, David D. ; Xu, Le ; Mei, Yingwu ; Pasek, Daniel A. ; Meissner, Gerhard ; Dokholyan, Nikolay. / Ion-pulling simulations provide insights into the mechanisms of channel opening of the skeletal muscle ryanodine receptor. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 31. pp. 12947-12958.
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abstract = "The type 1 ryanodine receptor (RyR1) mediates Ca2 release from the sarcoplasmic reticulum to initiate skeletal muscle contraction and is associated with muscle diseases, malignant hyperthermia, and central core disease. To better understand RyR1 channel function, we investigated the molecular mechanisms of channel gating and ion permeation. An adequate model of channel gating requires accurate, high-resolution models of both open and closed states of the channel. To this end, we generated an open-channel RyR1 model using molecular simulations to pull Ca2 through the pore constriction site of a closed-channel RyR1 structure determined at 3.8-{\AA} resolution. Importantly, we find that our open-channel model is consistent with the RyR1 and cardiac RyR (RyR2) open-channel structures reported while this paper was in preparation. Both our model and the published structures show similar rotation of the upper portion of the pore-lining S6 helix away from the 4-fold channel axis and twisting of Ile-4937 at the channel constriction site out of the channel pore. These motions result in a minimum open-channel pore radius of 3 {\AA} formed by Gln-4933, rather than Ile-4937 in the closed-channel structure. We also present functional support for our model by mutations around the closed- and open-channel constriction sites (Gln-4933 and Ile-4937). Our results indicate that use of ion-pulling simulations produces a RyR1 open-channel model, which can provide insights into the mechanisms of channel opening complementing those from the structural data.",
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Ion-pulling simulations provide insights into the mechanisms of channel opening of the skeletal muscle ryanodine receptor. / Mowrey, David D.; Xu, Le; Mei, Yingwu; Pasek, Daniel A.; Meissner, Gerhard; Dokholyan, Nikolay.

In: Journal of Biological Chemistry, Vol. 292, No. 31, 01.01.2017, p. 12947-12958.

Research output: Contribution to journalArticle

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AU - Xu, Le

AU - Mei, Yingwu

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AU - Dokholyan, Nikolay

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