Ionizing radiation promotes CCL27 secretion from keratinocytes through the cross talk between TNF-α and ROS

Qian Zhang, Linlin Zhu, Gang Wang, Ye Zhao, Na Xiong, Hegang Bao, Wensen Jin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The skin-associated chemokine CCL27 and its receptor CCR10 mediate the immune response of skin-homing T cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. However, whether ionizing radiation increases the levels of CCL27 secretion still remains unclear. In HaCaT cells, a human keratinocyte cell line, CCL27 secretion was markedly increased after X-ray irradiation. We further found that irradiation boosted the generation of reactive oxygen species (ROS), which was concomitant with the release of tumor necrosis factor-alpha (TNF-α). Moreover, alteration of ROS in irradiated HaCaT cells correlated with TNF-α secretion, indicating a positive loop of TNF-α secretion and ROS generation. This positive loop regulated the secretion of CCL27 from irradiated cells. We therefore concluded that the cross talk between TNF-α and ROS after keratinocytes was exposed to radiation, triggered CCL27 secretion for subsequent inflammation response.

Original languageEnglish (US)
Article numbere21868
JournalJournal of Biochemical and Molecular Toxicology
Volume31
Issue number3
DOIs
StatePublished - Mar 1 2017

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Ionizing radiation
Ionizing Radiation
Keratinocytes
Reactive Oxygen Species
Tumor Necrosis Factor-alpha
Skin
Chemokine CCL27
CCR10 Receptors
Cells
Dermatitis
Irradiation
T-cells
Contact Dermatitis
Atopic Dermatitis
Psoriasis
Skin Diseases
X-Rays
Radiation
Inflammation
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Zhang, Qian ; Zhu, Linlin ; Wang, Gang ; Zhao, Ye ; Xiong, Na ; Bao, Hegang ; Jin, Wensen. / Ionizing radiation promotes CCL27 secretion from keratinocytes through the cross talk between TNF-α and ROS. In: Journal of Biochemical and Molecular Toxicology. 2017 ; Vol. 31, No. 3.
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abstract = "The skin-associated chemokine CCL27 and its receptor CCR10 mediate the immune response of skin-homing T cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. However, whether ionizing radiation increases the levels of CCL27 secretion still remains unclear. In HaCaT cells, a human keratinocyte cell line, CCL27 secretion was markedly increased after X-ray irradiation. We further found that irradiation boosted the generation of reactive oxygen species (ROS), which was concomitant with the release of tumor necrosis factor-alpha (TNF-α). Moreover, alteration of ROS in irradiated HaCaT cells correlated with TNF-α secretion, indicating a positive loop of TNF-α secretion and ROS generation. This positive loop regulated the secretion of CCL27 from irradiated cells. We therefore concluded that the cross talk between TNF-α and ROS after keratinocytes was exposed to radiation, triggered CCL27 secretion for subsequent inflammation response.",
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Ionizing radiation promotes CCL27 secretion from keratinocytes through the cross talk between TNF-α and ROS. / Zhang, Qian; Zhu, Linlin; Wang, Gang; Zhao, Ye; Xiong, Na; Bao, Hegang; Jin, Wensen.

In: Journal of Biochemical and Molecular Toxicology, Vol. 31, No. 3, e21868, 01.03.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ionizing radiation promotes CCL27 secretion from keratinocytes through the cross talk between TNF-α and ROS

AU - Zhang, Qian

AU - Zhu, Linlin

AU - Wang, Gang

AU - Zhao, Ye

AU - Xiong, Na

AU - Bao, Hegang

AU - Jin, Wensen

PY - 2017/3/1

Y1 - 2017/3/1

N2 - The skin-associated chemokine CCL27 and its receptor CCR10 mediate the immune response of skin-homing T cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. However, whether ionizing radiation increases the levels of CCL27 secretion still remains unclear. In HaCaT cells, a human keratinocyte cell line, CCL27 secretion was markedly increased after X-ray irradiation. We further found that irradiation boosted the generation of reactive oxygen species (ROS), which was concomitant with the release of tumor necrosis factor-alpha (TNF-α). Moreover, alteration of ROS in irradiated HaCaT cells correlated with TNF-α secretion, indicating a positive loop of TNF-α secretion and ROS generation. This positive loop regulated the secretion of CCL27 from irradiated cells. We therefore concluded that the cross talk between TNF-α and ROS after keratinocytes was exposed to radiation, triggered CCL27 secretion for subsequent inflammation response.

AB - The skin-associated chemokine CCL27 and its receptor CCR10 mediate the immune response of skin-homing T cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. However, whether ionizing radiation increases the levels of CCL27 secretion still remains unclear. In HaCaT cells, a human keratinocyte cell line, CCL27 secretion was markedly increased after X-ray irradiation. We further found that irradiation boosted the generation of reactive oxygen species (ROS), which was concomitant with the release of tumor necrosis factor-alpha (TNF-α). Moreover, alteration of ROS in irradiated HaCaT cells correlated with TNF-α secretion, indicating a positive loop of TNF-α secretion and ROS generation. This positive loop regulated the secretion of CCL27 from irradiated cells. We therefore concluded that the cross talk between TNF-α and ROS after keratinocytes was exposed to radiation, triggered CCL27 secretion for subsequent inflammation response.

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