Isolation and analysis of recombinant DNA molecules containing yeast DNA

Thomas D. Petes, James R. Broach, Pieter C. Wensink, Lynna M. Hereford, Gerald R. Fink, David Botstein

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Abstract

2500 recombinant plasmids containing insertions of yeast nuclear DNA have been cloned in Escherichia coli. It can be calculated that about 85% of the yeast genome is represented in this collection. The clones have been characterized by hybridization to purified RNA species. Of the 2000 clones examined, 75 contain insertions of yeast ribosomal DNA, 201 contain insertions of yeast tRNA genes, and 26 contain DNA sequences that are complementary to abundant mRNA species.

Original languageEnglish (US)
Pages (from-to)37-49
Number of pages13
JournalGene
Volume4
Issue number1
DOIs
Publication statusPublished - Sep 1978

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All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Petes, T. D., Broach, J. R., Wensink, P. C., Hereford, L. M., Fink, G. R., & Botstein, D. (1978). Isolation and analysis of recombinant DNA molecules containing yeast DNA. Gene, 4(1), 37-49. https://doi.org/10.1016/0378-1119(78)90013-6