Isolation and characterization of a 30 kDa membrane glycoprotein from human stratum corneum

Shu-jen Chen, Srinivasan Rajaraman, Joanne Miller, Gulgun D. Kalmaz, Miriam M. Brysk

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Using iodinated concanavalin A in conjunction with gel electrophoresis, we have identified a 30 kDa glycoprotein in the stratum corneum of human skin. We isolated this glycoprotein by extraction in nonionic detergent, affinity chromatography and preparative gel electrophoresis. It binds to concanavalin A but not to three other lectins. The purified glycoprotein migrates at 30 kDa whether or not reducing agents are present. It is rich in histidine and lysine, but lacks arginine, proline, tyrosine and methionine. It is clearly distinct from fillaggrin. We prepared a monospecific polyclonal antibody to this glycoprotein and localized it by immuno-histochemistry exclusively to the cell membrane of corneocytes. We postulate that the glycoprotein may play a role in the cohesion and desquamation of corneocytes.

Original languageEnglish (US)
Pages (from-to)375-382
Number of pages8
JournalBBA - General Subjects
Volume881
Issue number3
DOIs
StatePublished - May 2 1986

Fingerprint

Membrane Glycoproteins
Cornea
Glycoproteins
Concanavalin A
Electrophoresis
Gels
Affinity chromatography
Reducing Agents
Cell membranes
Affinity Chromatography
Histidine
Lectins
Proline
Methionine
Detergents
Lysine
Tyrosine
Arginine
Skin
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Chen, Shu-jen ; Rajaraman, Srinivasan ; Miller, Joanne ; Kalmaz, Gulgun D. ; Brysk, Miriam M. / Isolation and characterization of a 30 kDa membrane glycoprotein from human stratum corneum. In: BBA - General Subjects. 1986 ; Vol. 881, No. 3. pp. 375-382.
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Isolation and characterization of a 30 kDa membrane glycoprotein from human stratum corneum. / Chen, Shu-jen; Rajaraman, Srinivasan; Miller, Joanne; Kalmaz, Gulgun D.; Brysk, Miriam M.

In: BBA - General Subjects, Vol. 881, No. 3, 02.05.1986, p. 375-382.

Research output: Contribution to journalArticle

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