Isolation and characterization of canine free secretory component

R. E. Thompson, H. Y. Reynolds, M. J. Waxdal

Research output: Contribution to journalArticle

Abstract

Free secretory component (FSC) was isolated for structural analysis from canine colostrum, using a combination of immunoabsorbants, gel filtration and ion exchange chromatography. The yield of FSC from one liter of colostrum was approximately 100 mg. With alkaline urea and sodium dodecyl sulfate (SDS) disc gel electrophoresis, FSC migrated as a single band with a corresponding mol wt (MW) of 77,500 daltons ± 6500. The carbohydrate content of FSC was 19% by weight (6% neutral hexose, 6.4% glucosamine and 6.6% galactosamine). The amino acid composition of FSC was similar to those reported for the human rabbit and cow. The amino acid sequence for the 12 NH2 terminal residues of FSC was: Lys-Ser-Pro-Ile-Phe-Gly-Pro-Glu-Glu-Val-Asn-Ile. The first 10 residues of this sequence was identical to that of human and bovine FSC. Canine FSC was further subjected to cyanogen bromide cleavage and the products were analyzed by SDS gel electrophoresis. Two bands were observed with estimated MW's of 55,000 daltons ± 5000 and 17,000 ± 1500. Prior reduction of the larger band with 2 mercaptoethanol indicated it was composed of several small fragments joined by disulfide bonds, whereas the smaller bands appeared to be a single fragment. Further analysis of these fragments may help elucidate the attachment site of secretory component to the alpha chain of colostral IgA.

Original languageEnglish (US)
Number of pages1
JournalFederation Proceedings
Volume34
Issue number3
StatePublished - Jan 1 1975

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Secretory Component
Canidae
Colostrum
Sodium Dodecyl Sulfate
Gels
Disc Electrophoresis
Secretory Immunoglobulin A
Cyanogen Bromide
Galactosamine
Hexoses
Mercaptoethanol
Glucosamine
Ion Exchange Chromatography
Disulfides
Gel Chromatography
Urea
Electrophoresis
Amino Acid Sequence
Carbohydrates
Rabbits

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Thompson, R. E., Reynolds, H. Y., & Waxdal, M. J. (1975). Isolation and characterization of canine free secretory component. Federation Proceedings, 34(3).
Thompson, R. E. ; Reynolds, H. Y. ; Waxdal, M. J. / Isolation and characterization of canine free secretory component. In: Federation Proceedings. 1975 ; Vol. 34, No. 3.
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Thompson, RE, Reynolds, HY & Waxdal, MJ 1975, 'Isolation and characterization of canine free secretory component', Federation Proceedings, vol. 34, no. 3.

Isolation and characterization of canine free secretory component. / Thompson, R. E.; Reynolds, H. Y.; Waxdal, M. J.

In: Federation Proceedings, Vol. 34, No. 3, 01.01.1975.

Research output: Contribution to journalArticle

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Thompson RE, Reynolds HY, Waxdal MJ. Isolation and characterization of canine free secretory component. Federation Proceedings. 1975 Jan 1;34(3).