Isolation and characterization of human breast cancer cells overexpressing S-adenosylmethionine decarboxylase

Andrea Manni, Betty Badger, Rhea Grove, Susan Kunselman, Laurence Demers

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We report the first successful isolation and initial characterization of S-adenosylmethionine decarboxylase (SAMDC)-overexpressing cells using a transfection approach. Stably transfected MCF-7 breast cancer cells overproducing SAMDC (~5-fold) manifested reduced ornithine decarboxylase while levels of N′-spermidine/spermine acetyltransferase were variably increased. Analysis of cellular polyamine profile showed that spermine was selectively increased (~80%), while spermidine and putrescine levels were reduced (~48% and ~15% of control, respectively). Since SAMDC-overexpressing clones exhibited increased clonogenicity in soft agar, our data suggest that spermine may be selectively involved in conferring a more invasive phenotype to breast cancer cells.

Original languageEnglish (US)
Pages (from-to)23-28
Number of pages6
JournalCancer Letters
Volume95
Issue number1-2
DOIs
StatePublished - Aug 16 1995

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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