Isolation and characterization of microsatellite loci in Alasmidonta heterodon (Bivalvia: Unionidae)

Kristine M. Shaw, Timothy Lee King, William A. Lellis, Michael S. Eackles

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We developed 13 species-specific microsatellite markers for the federally endangered Atlantic slope unionid Alasmidonta heterodon. Four to 18 alleles per locus were observed among 30 individuals. Observed heterozygosity throughout the loci ranged from 26.9 to 86.2% and averaged 63.6%. Estimates of individual pairwise genetic distances indicated that levels of genetic diversity among loci were sufficient to produce unique multilocus genotypes for all animals surveyed. Randomization tests showed that genotypes for this collection were consistent with Hardy-Weinberg expectations, and no significant linkage disequilibrium was observed between loci. These loci therefore appear suitable for population surveys, kinship assessment and other such applications.

Original languageEnglish (US)
Pages (from-to)365-367
Number of pages3
JournalMolecular Ecology Notes
Volume6
Issue number2
DOIs
StatePublished - Jun 1 2006

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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