Fifteen tetranucleotide microsatellite loci were identified and characterized for spotted salamanders (Ambystoma maculatum) collected from three vernal pools in the southeastern USA. These markers revealed a high degree of genetic diversity (7-32 alleles per locus), heterozygosity (31.6-86.3%) and allelic heterogeneity (91% of comparisons were statistically significant). Considerable differentiation among populations was observed as genetic distances (chord) ranged between 0.50 and 0.65, and all FST values (0.08-0.14) were statistically significant. Moreover, genotypic assignment tests correctly classified all individuals to their respective collection. These markers should prove useful for investigating fine-scale population structure and mating system.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)