Isolation and characterization of novel tetranucleotide microsatellite DNA markers for the spotted salamander, Ambystoma maculatum

S. E. Julian, Timothy Lee King, W. K. Savage

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Fifteen tetranucleotide microsatellite loci were identified and characterized for spotted salamanders (Ambystoma maculatum) collected from three vernal pools in the southeastern USA. These markers revealed a high degree of genetic diversity (7-32 alleles per locus), heterozygosity (31.6-86.3%) and allelic heterogeneity (91% of comparisons were statistically significant). Considerable differentiation among populations was observed as genetic distances (chord) ranged between 0.50 and 0.65, and all FST values (0.08-0.14) were statistically significant. Moreover, genotypic assignment tests correctly classified all individuals to their respective collection. These markers should prove useful for investigating fine-scale population structure and mating system.

Original languageEnglish (US)
Pages (from-to)7-9
Number of pages3
JournalMolecular Ecology Notes
Volume3
Issue number1
DOIs
StatePublished - Mar 1 2003

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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