Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: Genomic complexity and molecular evolution of the gene

J. Yun Tso, Xiao Hong Sun, Teh Hui Kao, Kimberly S. Reece, Ray Wu

Research output: Contribution to journalArticle

1725 Citations (Scopus)

Abstract

Full length cDns encoding the glycolyti enzme glyceraldehyde-3-phosphate dehyrogense (GAPDH)fron rat man have been in both genomes based on genmic blot hybridization analysis. Only one functional gene product is know. Result fron genomic library screeings suggest that are 300-400 copies of these sequences in the rat genome and approximately 100 in the human genome, Some of these realted sequences have been shown to be thesepseudogenes indicating that some pseudogenes are transcrined. Rat and human cDNA are 89% homologous in the coding region, and 76% homologous in the frist 100 base paris of the 3' -noncoding region. Comparison of these two cDNA sequences with those of the chicken, Drosophils and yeast genes allows analysis of the evolution of the GAPDH genes in detail.

Original languageEnglish (US)
Pages (from-to)2485-2502
Number of pages18
JournalNucleic acids research
Volume13
Issue number7
DOIs
StatePublished - Apr 11 1985

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Glyceraldehyde-3-Phosphate Dehydrogenases
Molecular Evolution
Glyceraldehyde 3-Phosphate
Complementary DNA
Genome
Genes
Pseudogenes
Genomic Library
Paris
Human Genome
Chickens
Yeasts

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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abstract = "Full length cDns encoding the glycolyti enzme glyceraldehyde-3-phosphate dehyrogense (GAPDH)fron rat man have been in both genomes based on genmic blot hybridization analysis. Only one functional gene product is know. Result fron genomic library screeings suggest that are 300-400 copies of these sequences in the rat genome and approximately 100 in the human genome, Some of these realted sequences have been shown to be thesepseudogenes indicating that some pseudogenes are transcrined. Rat and human cDNA are 89{\%} homologous in the coding region, and 76{\%} homologous in the frist 100 base paris of the 3' -noncoding region. Comparison of these two cDNA sequences with those of the chicken, Drosophils and yeast genes allows analysis of the evolution of the GAPDH genes in detail.",
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Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs : Genomic complexity and molecular evolution of the gene. / Tso, J. Yun; Sun, Xiao Hong; Kao, Teh Hui; Reece, Kimberly S.; Wu, Ray.

In: Nucleic acids research, Vol. 13, No. 7, 11.04.1985, p. 2485-2502.

Research output: Contribution to journalArticle

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