Genomic and cDNA clones encoding the Drosophila melanogaster α-subunit of translational initiation factor 2 (eIF-2α) were isolated. The D. melanogaster eIF-2α gene encodes a 341 amino-acid (aa) protein that shares 57 and 44% identity to its human and yeast homologues, respectively. The regulatory phosphorylation site at Ser50 is embedded in a segment of 19 conserved aa residues. Analysis of the genomic DNA and cDNA clones indicated that eIF-2α is a single-copy gene and its coding region is interrupted by a 260-bp intron. The D. melanogaster eIF-2α mRNA is 1350 nt in length and is expressed throughout development.
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