Isolation and identification of highly pathogenic avian influenza virus subtype H5N1 in peafowl (Pavo cristatus)

Mahmoud Moussa Ismail, Owais Ahmed Khan, Giovanni Cattoli, Huaguang Lu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An outbreak of highly pathogenic avian influenza (HPAI) virus subtype H5N1 was first diagnosed in a "backyard" flock of peafowl (Pavo cristatus) raised on palace premises in the Kingdom of Saudi Arabia in December 3, 2007. The flock consisted of 40 peafowl, and their ages ranged from 3 to 5 years old. Affected birds suffered from depression, anorexia, and white diarrhea. Four dead birds were submitted for HPAI diagnosis at the Central Veterinary Diagnostic Laboratory in Riyadh. Brain and liver tissues and tracheal and cloacal swabs were taken from the dead birds and processed for a real-time reverse transcriptase (RT)-PCR test and virus isolation in specific-pathogen-free embryonating chicken eggs. The H5N1 subtype of avian influenza virus was isolated from the four dead birds and identified by a real-time RT-PCR before and after egg inoculation. The virus isolates were characterized as HPAI H5N1 virus by sequencing analysis. Phylogenetic comparisons revealed that the H5N1 viruses isolated from peafowl belong to the genetic clade 2.2 according to the World Health Organization nomenclature. The peafowl H5N1 virus falls into 2.2.2 sublineage II and clusters with the H5N1 viruses isolated from poultry in Saudi Arabia in 2007-08

Original languageEnglish (US)
Pages (from-to)357-360
Number of pages4
JournalAvian diseases
Volume54
Issue numberSUPPL. 1
DOIs
StatePublished - Mar 1 2010

Fingerprint

H5N1 Subtype Influenza A Virus
peafowl
Influenza in Birds
Orthomyxoviridae
Influenza A virus
Birds
Saudi Arabia
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
birds
avian influenza
Viruses
Specific Pathogen-Free Organisms
flocks
Anorexia
Poultry
reverse transcriptase polymerase chain reaction
Terminology
Eggs
Disease Outbreaks

All Science Journal Classification (ASJC) codes

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

Ismail, Mahmoud Moussa ; Khan, Owais Ahmed ; Cattoli, Giovanni ; Lu, Huaguang. / Isolation and identification of highly pathogenic avian influenza virus subtype H5N1 in peafowl (Pavo cristatus). In: Avian diseases. 2010 ; Vol. 54, No. SUPPL. 1. pp. 357-360.
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Isolation and identification of highly pathogenic avian influenza virus subtype H5N1 in peafowl (Pavo cristatus). / Ismail, Mahmoud Moussa; Khan, Owais Ahmed; Cattoli, Giovanni; Lu, Huaguang.

In: Avian diseases, Vol. 54, No. SUPPL. 1, 01.03.2010, p. 357-360.

Research output: Contribution to journalArticle

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