Isolation and Identification of the Follicular Microbiome: Implications for Acne Research

Jacob B. Hall, Zhaoyuan Cong, Yuka Imamura, Brian A. Kidd, Joel T. Dudley, Diane Thiboutot, Amanda Nelson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Our understanding of the microbiome and the role of Propionibacterium acnes in skin homeostasis and acne pathogenesis is evolving. Multiple methods for sampling and identifying the skin's microbiome exist, and understanding the differences between the abilities of various methods to characterize the microbial landscape is warranted. This study compared the microbial diversity of samples obtained from the cheeks of 20 volunteers, collected by surface swab, pore strips, and cyanoacrylate glue follicular biopsy, all sequenced with 16S rRNA sequencing (V1–V3) and whole-genome metagenomic sequencing. The sequencing method of choice influenced the detection of microbial profiles as whole-genome sequencing captured more species diversity, including viruses, compared with 16S sequencing. The relative abundance of bacterial or fungal species and overall diversity did not differ between sampling methods. However, the viral composition of the skin's surface is unique compared with the follicle, suggesting distinct viral niches within the skin. P. acnes bacteria, ribotypes, and bacteriophages were identified equally by all sampling methods indicating that the sampling method, whether for the skin's surface or follicle, does not impact P. acnes-related characterization and that all may be equally useful for acne-related research studies.

Original languageEnglish (US)
Pages (from-to)2033-2040
Number of pages8
JournalJournal of Investigative Dermatology
Volume138
Issue number9
DOIs
StatePublished - Sep 1 2018

Fingerprint

Microbiota
Acne Vulgaris
Skin
Propionibacterium acnes
Sampling
Research
Genes
Cyanoacrylates
Bacteriophages
Genome
Glues
Biopsy
Biodiversity
Ribotyping
Metagenomics
Viruses
Cheek
Bacteria
Adhesives
Volunteers

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

@article{9666eb3d3d674692b9ec035348f4d3dd,
title = "Isolation and Identification of the Follicular Microbiome: Implications for Acne Research",
abstract = "Our understanding of the microbiome and the role of Propionibacterium acnes in skin homeostasis and acne pathogenesis is evolving. Multiple methods for sampling and identifying the skin's microbiome exist, and understanding the differences between the abilities of various methods to characterize the microbial landscape is warranted. This study compared the microbial diversity of samples obtained from the cheeks of 20 volunteers, collected by surface swab, pore strips, and cyanoacrylate glue follicular biopsy, all sequenced with 16S rRNA sequencing (V1–V3) and whole-genome metagenomic sequencing. The sequencing method of choice influenced the detection of microbial profiles as whole-genome sequencing captured more species diversity, including viruses, compared with 16S sequencing. The relative abundance of bacterial or fungal species and overall diversity did not differ between sampling methods. However, the viral composition of the skin's surface is unique compared with the follicle, suggesting distinct viral niches within the skin. P. acnes bacteria, ribotypes, and bacteriophages were identified equally by all sampling methods indicating that the sampling method, whether for the skin's surface or follicle, does not impact P. acnes-related characterization and that all may be equally useful for acne-related research studies.",
author = "Hall, {Jacob B.} and Zhaoyuan Cong and Yuka Imamura and Kidd, {Brian A.} and Dudley, {Joel T.} and Diane Thiboutot and Amanda Nelson",
year = "2018",
month = "9",
day = "1",
doi = "10.1016/j.jid.2018.02.038",
language = "English (US)",
volume = "138",
pages = "2033--2040",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "9",

}

Isolation and Identification of the Follicular Microbiome : Implications for Acne Research. / Hall, Jacob B.; Cong, Zhaoyuan; Imamura, Yuka; Kidd, Brian A.; Dudley, Joel T.; Thiboutot, Diane; Nelson, Amanda.

In: Journal of Investigative Dermatology, Vol. 138, No. 9, 01.09.2018, p. 2033-2040.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation and Identification of the Follicular Microbiome

T2 - Implications for Acne Research

AU - Hall, Jacob B.

AU - Cong, Zhaoyuan

AU - Imamura, Yuka

AU - Kidd, Brian A.

AU - Dudley, Joel T.

AU - Thiboutot, Diane

AU - Nelson, Amanda

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Our understanding of the microbiome and the role of Propionibacterium acnes in skin homeostasis and acne pathogenesis is evolving. Multiple methods for sampling and identifying the skin's microbiome exist, and understanding the differences between the abilities of various methods to characterize the microbial landscape is warranted. This study compared the microbial diversity of samples obtained from the cheeks of 20 volunteers, collected by surface swab, pore strips, and cyanoacrylate glue follicular biopsy, all sequenced with 16S rRNA sequencing (V1–V3) and whole-genome metagenomic sequencing. The sequencing method of choice influenced the detection of microbial profiles as whole-genome sequencing captured more species diversity, including viruses, compared with 16S sequencing. The relative abundance of bacterial or fungal species and overall diversity did not differ between sampling methods. However, the viral composition of the skin's surface is unique compared with the follicle, suggesting distinct viral niches within the skin. P. acnes bacteria, ribotypes, and bacteriophages were identified equally by all sampling methods indicating that the sampling method, whether for the skin's surface or follicle, does not impact P. acnes-related characterization and that all may be equally useful for acne-related research studies.

AB - Our understanding of the microbiome and the role of Propionibacterium acnes in skin homeostasis and acne pathogenesis is evolving. Multiple methods for sampling and identifying the skin's microbiome exist, and understanding the differences between the abilities of various methods to characterize the microbial landscape is warranted. This study compared the microbial diversity of samples obtained from the cheeks of 20 volunteers, collected by surface swab, pore strips, and cyanoacrylate glue follicular biopsy, all sequenced with 16S rRNA sequencing (V1–V3) and whole-genome metagenomic sequencing. The sequencing method of choice influenced the detection of microbial profiles as whole-genome sequencing captured more species diversity, including viruses, compared with 16S sequencing. The relative abundance of bacterial or fungal species and overall diversity did not differ between sampling methods. However, the viral composition of the skin's surface is unique compared with the follicle, suggesting distinct viral niches within the skin. P. acnes bacteria, ribotypes, and bacteriophages were identified equally by all sampling methods indicating that the sampling method, whether for the skin's surface or follicle, does not impact P. acnes-related characterization and that all may be equally useful for acne-related research studies.

UR - http://www.scopus.com/inward/record.url?scp=85047442461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047442461&partnerID=8YFLogxK

U2 - 10.1016/j.jid.2018.02.038

DO - 10.1016/j.jid.2018.02.038

M3 - Article

C2 - 29548797

AN - SCOPUS:85047442461

VL - 138

SP - 2033

EP - 2040

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 9

ER -