Abstract
Previous research in our laboratory has shown basic fibroblast growth factor (bFGF) to be a permissive mitogen for isolated avian growth plate chondrocytes. The present study was conducted to determine whether bFGF is present in avian growth plate and, if present, to determine its localization within the tissue. Immunohistochemical studies revealed that bFGF is present in the resting proliferative and hypertrophic calcifying zones of the growth plate but is absent from the prehypertrophic zone. Basic FGF appears to be associated with the extracellular matrix of the proliferative zone, but it is predominantly intracellular in the hypertrophic and mineralizing zone chondrocytes. Partial purification of cartilage‐derived bFGF was performed on crude extracts of cartilage using heparin‐Sepharose affinity chromatography. The presence of bFGF in the heparin‐Sepharose column fractions was confirmed by immunoblotting and radioimmunoassay. Furthermore, western blot analysis of the extracts showed multiple protein bands having bFGF immunoreactivity, in the molecular weight range 14.4–18 kD. The data support the hypothesis that bFGF has a dual role in the growth plate. In the proliferative zone it acts as a chondrocyte mitogen, whereas when released from terminal hypertrophic chondrocytes, bFGF may serve as a chemotactic signal for metaphyseal blood vessel proliferation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1737-1744 |
| Number of pages | 8 |
| Journal | Journal of Bone and Mineral Research |
| Volume | 9 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jan 1 1994 |
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All Science Journal Classification (ASJC) codes
- Endocrinology, Diabetes and Metabolism
- Orthopedics and Sports Medicine
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Isolation and localization of basic fibroblast growth factor‐immunoreactive substance in the epiphyseal growth plate. / Twal, W. O.; Vasilatos-Younken, Regina; Gay, C. V.; Leach, R. M.
In: Journal of Bone and Mineral Research, Vol. 9, No. 11, 01.01.1994, p. 1737-1744.Research output: Contribution to journal › Article
TY - JOUR
T1 - Isolation and localization of basic fibroblast growth factor‐immunoreactive substance in the epiphyseal growth plate
AU - Twal, W. O.
AU - Vasilatos-Younken, Regina
AU - Gay, C. V.
AU - Leach, R. M.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - Previous research in our laboratory has shown basic fibroblast growth factor (bFGF) to be a permissive mitogen for isolated avian growth plate chondrocytes. The present study was conducted to determine whether bFGF is present in avian growth plate and, if present, to determine its localization within the tissue. Immunohistochemical studies revealed that bFGF is present in the resting proliferative and hypertrophic calcifying zones of the growth plate but is absent from the prehypertrophic zone. Basic FGF appears to be associated with the extracellular matrix of the proliferative zone, but it is predominantly intracellular in the hypertrophic and mineralizing zone chondrocytes. Partial purification of cartilage‐derived bFGF was performed on crude extracts of cartilage using heparin‐Sepharose affinity chromatography. The presence of bFGF in the heparin‐Sepharose column fractions was confirmed by immunoblotting and radioimmunoassay. Furthermore, western blot analysis of the extracts showed multiple protein bands having bFGF immunoreactivity, in the molecular weight range 14.4–18 kD. The data support the hypothesis that bFGF has a dual role in the growth plate. In the proliferative zone it acts as a chondrocyte mitogen, whereas when released from terminal hypertrophic chondrocytes, bFGF may serve as a chemotactic signal for metaphyseal blood vessel proliferation.
AB - Previous research in our laboratory has shown basic fibroblast growth factor (bFGF) to be a permissive mitogen for isolated avian growth plate chondrocytes. The present study was conducted to determine whether bFGF is present in avian growth plate and, if present, to determine its localization within the tissue. Immunohistochemical studies revealed that bFGF is present in the resting proliferative and hypertrophic calcifying zones of the growth plate but is absent from the prehypertrophic zone. Basic FGF appears to be associated with the extracellular matrix of the proliferative zone, but it is predominantly intracellular in the hypertrophic and mineralizing zone chondrocytes. Partial purification of cartilage‐derived bFGF was performed on crude extracts of cartilage using heparin‐Sepharose affinity chromatography. The presence of bFGF in the heparin‐Sepharose column fractions was confirmed by immunoblotting and radioimmunoassay. Furthermore, western blot analysis of the extracts showed multiple protein bands having bFGF immunoreactivity, in the molecular weight range 14.4–18 kD. The data support the hypothesis that bFGF has a dual role in the growth plate. In the proliferative zone it acts as a chondrocyte mitogen, whereas when released from terminal hypertrophic chondrocytes, bFGF may serve as a chemotactic signal for metaphyseal blood vessel proliferation.
UR - http://www.scopus.com/inward/record.url?scp=0027948601&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027948601&partnerID=8YFLogxK
U2 - 10.1002/jbmr.5650091110
DO - 10.1002/jbmr.5650091110
M3 - Article
C2 - 7863825
AN - SCOPUS:0027948601
VL - 9
SP - 1737
EP - 1744
JO - Journal of Bone and Mineral Research
JF - Journal of Bone and Mineral Research
SN - 0884-0431
IS - 11
ER -