Isolation and profiling of plasma microRNAs: Biomarkers for asthma and allergic rhinitis

Ronaldo P. Panganiban, Kristin A. Lambert, Man Hsun Hsu, Zoe Laryea, Faoud T. Ishmael

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Chronic inflammatory diseases can be particularly challenging to diagnose and characterize, as inflammatory changes in tissue may not be present in blood. There is a crucial need to develop non-invasive biomarkers that would be useful in diagnosing disease and selecting medical therapies. For example, there are no blood tests to diagnose asthma, a common inflammatory lung disease. MicroRNA (miRNA) expression profiling in blood is emerging as a potentially sensitive and useful biomarker of many diseases. In particular, we have characterized a cost-effective PCR-based array technology to measure and profile circulating miRNAs in the plasma of patients with allergic rhinitis and asthma. Here, we describe the methods to isolate, quantify, and analyze miRNAs in the plasma of human subjects as well as ways to determine their diagnostic utility.

Original languageEnglish (US)
Pages (from-to)48-54
Number of pages7
JournalMethods
Volume152
DOIs
StatePublished - Jan 1 2019

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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