Isolation and purification of perfluorodecanoic and perfluorooctanoic acids from rat tissues

John Patrick Vanden Heuvel, Marc J. Van Rafelghem, Lawrence A. Menahan, Richard E. Peterson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A procedure for the extraction, separation, and isolation of perfluorodecanoic and perfluorooctanoic acids from biological samples is described. The use of conventional lipid extraction procedures leads to substantial loss of the perfluorinated fatty acids added to tissue. The presence of sulfuric acid in aqueous saline during phase partitioning is essential for the recovery of perfluorodecanoic and perfluorooctanoic acids in the organic phase following their extraction from tissue. The perfluorinated fatty acids are co-eluted with simple lipids from silica gel columns using diethyl ether/trifluoroacetic acid (100:1, v/v). Simple lipids are separated by thin layer chromatography. By substituting trifluoroacetic acid for acetic acid in the developing solvents, perfluorodecanoic and perfluorooctanoic acids migrate with other free fatty acids.

Original languageEnglish (US)
Pages (from-to)526-531
Number of pages6
JournalLipids
Volume24
Issue number6
DOIs
StatePublished - Jun 1 1989

Fingerprint

perfluorooctanoic acid
Purification
Rats
Trifluoroacetic Acid
Tissue
Lipids
Fatty Acids
Thin layer chromatography
Silica Gel
Thin Layer Chromatography
Nonesterified Fatty Acids
Acetic Acid
Ether
Recovery
perfluorodecanoic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Vanden Heuvel, John Patrick ; Van Rafelghem, Marc J. ; Menahan, Lawrence A. ; Peterson, Richard E. / Isolation and purification of perfluorodecanoic and perfluorooctanoic acids from rat tissues. In: Lipids. 1989 ; Vol. 24, No. 6. pp. 526-531.
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Isolation and purification of perfluorodecanoic and perfluorooctanoic acids from rat tissues. / Vanden Heuvel, John Patrick; Van Rafelghem, Marc J.; Menahan, Lawrence A.; Peterson, Richard E.

In: Lipids, Vol. 24, No. 6, 01.06.1989, p. 526-531.

Research output: Contribution to journalArticle

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T1 - Isolation and purification of perfluorodecanoic and perfluorooctanoic acids from rat tissues

AU - Vanden Heuvel, John Patrick

AU - Van Rafelghem, Marc J.

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AU - Peterson, Richard E.

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AB - A procedure for the extraction, separation, and isolation of perfluorodecanoic and perfluorooctanoic acids from biological samples is described. The use of conventional lipid extraction procedures leads to substantial loss of the perfluorinated fatty acids added to tissue. The presence of sulfuric acid in aqueous saline during phase partitioning is essential for the recovery of perfluorodecanoic and perfluorooctanoic acids in the organic phase following their extraction from tissue. The perfluorinated fatty acids are co-eluted with simple lipids from silica gel columns using diethyl ether/trifluoroacetic acid (100:1, v/v). Simple lipids are separated by thin layer chromatography. By substituting trifluoroacetic acid for acetic acid in the developing solvents, perfluorodecanoic and perfluorooctanoic acids migrate with other free fatty acids.

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