Isolation characterization of a novel gene induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rat liver

Ornella Selmin, George W. Lucier, George C. Clark, Angelika M. Tritscher, John P. Vanden Heuvel, Jonathan A. Gastel, Nigel J. Walker, Thomas R. Sutter, Douglas A. Bell

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103 Scopus citations

Abstract

The differential display technique was used to identify genes whose expression was regulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Expression of a novel sequence was up-regulated in a dose-dependent fashion in liver of Sprague-Dawley male rats exposed to both chronic and acute treatment with TCDD, as measured by densitometry of Northern blot analyses (P < 0.01). A rapid amplification of cDNA ends (RACE) procedure was used to isolate a 1.8 kb cDNA from a rat liver cDNA preparation. This cloned cDNA, called 25-Dx, was sequenced and found to encode a peptide of 223 amino acids. In control rats, the 25-Dx gene was expressed at high levels in lung and liver. A hydrophobic domain of 14 residues followed by a proline-rich domain, both located in the N-terminal region, showed 71% homology with the transmembrane domain of the precursor for the interleukin-6 receptor and a conserved consensus sequence found in the cytokine/growth factor/prolactin receptor superfamily respectively.

Original languageEnglish (US)
Pages (from-to)2609-2615
Number of pages7
JournalCarcinogenesis
Volume17
Issue number12
DOIs
StatePublished - 1996

All Science Journal Classification (ASJC) codes

  • Cancer Research

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