Isolation, cloning, and characterization of a novel rat lung zinc finger gene, RLZF-Y

Sinisa Dovat, Kirk A. Gilbert, Lidija Petrovic-Dovat, D. Eugene Rannels

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Zinc-finger (ZF) proteins are widely distributed. The current study reports isolation, cloning and characterization of a novel ZF gene, RLZF-Y. Total RNA from rat lung was reverse transcribed. The 5' and 3' ends were isolated by rapid amplification of cDNA ends (RACE) using primers derived from a previously isolated partial clone. RACE products of 1.5 and 1.1 kb were cloned and sequenced. Identical overlapping sequence of 70 base pairs confirmed representation of the same cDNA approximately 2.5 kb in length. Probes derived from both 5' RACE and 3' RACE products independently hybridized to a 2.5 kb mRNA from rat lung. RLZF-Y mRNA is expressed in lung, brain, heart and kidney; expression is low in liver. Predicted amino acid sequence analysis defined three regions of similarity to known C2H2 ZF proteins: a region containing seven ZF structures characteristic of the Kruppel-like subfamily of ZF genes; a region with sequence similarity to the Kruppel-associated box A (KRAB-A) domain at the amino end; an amino-terminal leucine-rich region (LeR) adjacent to KRAB-A. The presence of KRAB-A and the adjacent LeR implies RLZF-Y protein may function as a transcriptional repressor.

Original languageEnglish (US)
Pages (from-to)380-388
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1442
Issue number2-3
DOIs
StatePublished - Nov 8 1998

Fingerprint

Cloning
Zinc Fingers
Zinc
Rats
Organism Cloning
Complementary DNA
Genes
Amplification
Lung
Leucine
Messenger RNA
Proteins
Protein Sequence Analysis
Base Pairing
Liver
Brain
Clone Cells
RNA
Kidney
Amino Acids

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

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title = "Isolation, cloning, and characterization of a novel rat lung zinc finger gene, RLZF-Y",
abstract = "Zinc-finger (ZF) proteins are widely distributed. The current study reports isolation, cloning and characterization of a novel ZF gene, RLZF-Y. Total RNA from rat lung was reverse transcribed. The 5' and 3' ends were isolated by rapid amplification of cDNA ends (RACE) using primers derived from a previously isolated partial clone. RACE products of 1.5 and 1.1 kb were cloned and sequenced. Identical overlapping sequence of 70 base pairs confirmed representation of the same cDNA approximately 2.5 kb in length. Probes derived from both 5' RACE and 3' RACE products independently hybridized to a 2.5 kb mRNA from rat lung. RLZF-Y mRNA is expressed in lung, brain, heart and kidney; expression is low in liver. Predicted amino acid sequence analysis defined three regions of similarity to known C2H2 ZF proteins: a region containing seven ZF structures characteristic of the Kruppel-like subfamily of ZF genes; a region with sequence similarity to the Kruppel-associated box A (KRAB-A) domain at the amino end; an amino-terminal leucine-rich region (LeR) adjacent to KRAB-A. The presence of KRAB-A and the adjacent LeR implies RLZF-Y protein may function as a transcriptional repressor.",
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Isolation, cloning, and characterization of a novel rat lung zinc finger gene, RLZF-Y. / Dovat, Sinisa; Gilbert, Kirk A.; Petrovic-Dovat, Lidija; Rannels, D. Eugene.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1442, No. 2-3, 08.11.1998, p. 380-388.

Research output: Contribution to journalArticle

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