Isolation of a cDNA clone encoding a high molecular weight precursor to a 6-kDa pulmonary surfactant-associated protein

K. A. Jacobs, D. S. Phelps, R. Steinbrink, J. Fisch, R. Kriz, L. Mitsock, J. P. Dougherty, H. W. Taeusch, J. Floros

Research output: Contribution to journalArticle

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Abstract

Mammalian surfactant is an incompletely defined mixture of lipids and associated proteins of molecular mass 35,000 Da and approximately 6,000 Da. Surfactant preparations which are highly effective in treating respiratory distress syndrome in premature infants lack the 35-kDa proteins, but contain the 6-kDa proteins. We isolated and partially sequenced one of these low molecular weight proteins from the lung lavage material of an alveolar proteinosis patient. Oligonucleotides deduced from the sequence were used as probes to isolate a human cDNA clone. The clone codes for a 42-kDa protein which contains the sequence of the 6-kDa protein. Messenger RNA coding for the 42-kDa protein was identified in human lung RNA by in vitro translation and immunoprecipitation of the translation products with an antiserum against purified bovine surfactant 6-kDa proteins. Immunoprecipitation of the 42-kDa primary translation product is inhibited by the presence of the bovine 6-kDa protein. These observations suggest a precursor-product relationship of the 42-kDa protein to one of the 6-kDa proteins.

Original languageEnglish (US)
Pages (from-to)9808-9811
Number of pages4
JournalJournal of Biological Chemistry
Volume262
Issue number20
StatePublished - Sep 17 1987

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Pulmonary Surfactant-Associated Proteins
Complementary DNA
Clone Cells
Molecular Weight
Molecular weight
Proteins
Surface-Active Agents
Immunoprecipitation
Molecular mass
Bronchoalveolar Lavage
Oligonucleotides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Jacobs, K. A. ; Phelps, D. S. ; Steinbrink, R. ; Fisch, J. ; Kriz, R. ; Mitsock, L. ; Dougherty, J. P. ; Taeusch, H. W. ; Floros, J. / Isolation of a cDNA clone encoding a high molecular weight precursor to a 6-kDa pulmonary surfactant-associated protein. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 20. pp. 9808-9811.
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Jacobs, KA, Phelps, DS, Steinbrink, R, Fisch, J, Kriz, R, Mitsock, L, Dougherty, JP, Taeusch, HW & Floros, J 1987, 'Isolation of a cDNA clone encoding a high molecular weight precursor to a 6-kDa pulmonary surfactant-associated protein', Journal of Biological Chemistry, vol. 262, no. 20, pp. 9808-9811.

Isolation of a cDNA clone encoding a high molecular weight precursor to a 6-kDa pulmonary surfactant-associated protein. / Jacobs, K. A.; Phelps, D. S.; Steinbrink, R.; Fisch, J.; Kriz, R.; Mitsock, L.; Dougherty, J. P.; Taeusch, H. W.; Floros, J.

In: Journal of Biological Chemistry, Vol. 262, No. 20, 17.09.1987, p. 9808-9811.

Research output: Contribution to journalArticle

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T1 - Isolation of a cDNA clone encoding a high molecular weight precursor to a 6-kDa pulmonary surfactant-associated protein

AU - Jacobs, K. A.

AU - Phelps, D. S.

AU - Steinbrink, R.

AU - Fisch, J.

AU - Kriz, R.

AU - Mitsock, L.

AU - Dougherty, J. P.

AU - Taeusch, H. W.

AU - Floros, J.

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N2 - Mammalian surfactant is an incompletely defined mixture of lipids and associated proteins of molecular mass 35,000 Da and approximately 6,000 Da. Surfactant preparations which are highly effective in treating respiratory distress syndrome in premature infants lack the 35-kDa proteins, but contain the 6-kDa proteins. We isolated and partially sequenced one of these low molecular weight proteins from the lung lavage material of an alveolar proteinosis patient. Oligonucleotides deduced from the sequence were used as probes to isolate a human cDNA clone. The clone codes for a 42-kDa protein which contains the sequence of the 6-kDa protein. Messenger RNA coding for the 42-kDa protein was identified in human lung RNA by in vitro translation and immunoprecipitation of the translation products with an antiserum against purified bovine surfactant 6-kDa proteins. Immunoprecipitation of the 42-kDa primary translation product is inhibited by the presence of the bovine 6-kDa protein. These observations suggest a precursor-product relationship of the 42-kDa protein to one of the 6-kDa proteins.

AB - Mammalian surfactant is an incompletely defined mixture of lipids and associated proteins of molecular mass 35,000 Da and approximately 6,000 Da. Surfactant preparations which are highly effective in treating respiratory distress syndrome in premature infants lack the 35-kDa proteins, but contain the 6-kDa proteins. We isolated and partially sequenced one of these low molecular weight proteins from the lung lavage material of an alveolar proteinosis patient. Oligonucleotides deduced from the sequence were used as probes to isolate a human cDNA clone. The clone codes for a 42-kDa protein which contains the sequence of the 6-kDa protein. Messenger RNA coding for the 42-kDa protein was identified in human lung RNA by in vitro translation and immunoprecipitation of the translation products with an antiserum against purified bovine surfactant 6-kDa proteins. Immunoprecipitation of the 42-kDa primary translation product is inhibited by the presence of the bovine 6-kDa protein. These observations suggest a precursor-product relationship of the 42-kDa protein to one of the 6-kDa proteins.

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