Isolation of a Female-Emitted Sex Pheromone Component of the Fungus Gnat, Lycoriella ingenua, Attractive to Males

Stefanos S. Andreadis, Kevin R. Cloonan, Andrew J. Myrick, Haibin Chen, Thomas C. Baker

Research output: Contribution to journalArticle

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Abstract

Lycoriella ingenua Dufour (Diptera: Sciaridae) is acknowledged as the major pest species of the white button mushroom, Agaricus bisporus, throughout the world. Components of the female-produced sex pheromone of this species were identified previously as C15-C18n-alkanes, with the major component n-heptadecane, and shown to be attractive to L. mali. However, a subsequent report could not repeat this work. We reinvestigated the sex pheromone of this species by confirming that virgin females were attractive to males in a Y-tube bioassay and by collection of extracts from virgin females. Extracts were analyzed by gas chromatography coupled to electroantennographic detection, and by the less widely-used technique of gas chromatography coupled to a behavioral bioassay to detect compounds causing wing-fanning and copulatory abdomen curling in males. A single, behaviorally-active pheromone component was isolated and characterized by gas chromatography coupled to mass spectrometry. This component was definitively not n-heptadecane or any of the other C15-C19n-alkanes reported previously, but is proposed to be a sesquiterpene alcohol having analytical characteristics that closely matched those of reference germacradienols.

Original languageEnglish (US)
Pages (from-to)1127-1136
Number of pages10
JournalJournal of Chemical Ecology
Volume41
Issue number12
DOIs
StatePublished - Dec 1 2015

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All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

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