Isolation of avian pneumovirus from an outbreak of respiratory illness in Minnesota turkeys

Sagar M. Goyal, Shu Ju Chiang, Arshud M. Dar, K. V. Nagaraja, Daniel P. Shaw, David A. Halvorson, Vivek Kapur

Research output: Contribution to journalArticle

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Abstract

Antibodies to avian pneumovirus (APV) were first detected in Minnesota turkeys in 1997. Virus isolation was attempted on 32 samples (28 tracheal swabs, 4 pools of trachea and turbinates) that were positive for APV by reverse transcriptase polymerase chain reaction (RT-PCR). The cell cultures used were chicken embryo fibroblast (CEF), Vero cells, and QT-35 cells. Five virus isolates were obtained from these samples, and the identity of the isolates was confirmed by RT-PCR. Four isolates were obtained by inoculation of CEF cells, and 1 isolate was obtained in QT-35 cells after 3-7 blind passages in cell cultures. Vero cells did not yield any isolate on primary isolation; however, all 5 isolates could be adapted to grow in Vero cells following primary isolation in CEF or QT-35 cells. This is the first report of isolation of APV in Minnesota and also the first report of primary isolation of APV in QT-35 cells.

Original languageEnglish (US)
Pages (from-to)166-168
Number of pages3
JournalJournal of Veterinary Diagnostic Investigation
Volume12
Issue number2
DOIs
StatePublished - Jan 1 2000

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Metapneumovirus
Avian metapneumovirus
Disease Outbreaks
Vero Cells
Chickens
Embryonic Structures
Fibroblasts
Reverse Transcriptase Polymerase Chain Reaction
fibroblasts
cells
embryo (animal)
Cell Culture Techniques
chickens
Viruses
Turbinates
cell culture
reverse transcriptase polymerase chain reaction
Trachea
viruses
trachea (vertebrates)

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Goyal, Sagar M. ; Chiang, Shu Ju ; Dar, Arshud M. ; Nagaraja, K. V. ; Shaw, Daniel P. ; Halvorson, David A. ; Kapur, Vivek. / Isolation of avian pneumovirus from an outbreak of respiratory illness in Minnesota turkeys. In: Journal of Veterinary Diagnostic Investigation. 2000 ; Vol. 12, No. 2. pp. 166-168.
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Isolation of avian pneumovirus from an outbreak of respiratory illness in Minnesota turkeys. / Goyal, Sagar M.; Chiang, Shu Ju; Dar, Arshud M.; Nagaraja, K. V.; Shaw, Daniel P.; Halvorson, David A.; Kapur, Vivek.

In: Journal of Veterinary Diagnostic Investigation, Vol. 12, No. 2, 01.01.2000, p. 166-168.

Research output: Contribution to journalArticle

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