Isolation of candidate hybrid sterility 1 genes by cDNA selection in a 1.1 megabase pair region on mouse Chromosome 17

Zdenek Trachtulec, Monika Mňuková-Fajdelová, Renata M.J. Hamvas, Soña Gregorová, Werner E. Mayer, Hans R. Lehrach, Vladimir Vincek, Jiří Forejt, Jan Klein

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33 Scopus citations

Abstract

The Hybrid sterility 1 (Hst1) gene causes male infertility in crosses between certain inbred strains of the laboratory and wild mouse, Mus musculus. To identify the causative gene, we have searched YAC clones encompassing the Hst1 region for testis-expressed sequences, using the cDNA selection method. We isolated 12 non-overlapping cDNA clones, sequenced them, and placed them on a physical map based on the analysis of YAC clones and total genomic DNA. The cDNA clones map to ten loci. Three cDNA sequences correspond to the proteasome subunit C5 (locus Psmb1), ornithine decarboxylase (Odc-rs15), and pentazinc finger (Zfp91-rs1) transcripts. Three of the ten testis-expressed loci described in this report (D17Ph4e, Psmb1, and Zfp91-rsl) co-segregate with all Hst1 recombinants and, together with the Tbp gene, are therefore potential candidates for the Hst1 gene. The presented physical and genetic mapping data indicate there are no gross rearrangements distinguishing the Hstl(f) and Hstl(s) alleles.

Original languageEnglish (US)
Pages (from-to)312-316
Number of pages5
JournalMammalian Genome
Volume8
Issue number5
DOIs
StatePublished - 1997

All Science Journal Classification (ASJC) codes

  • Genetics

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