Isolation of ESTs from cacao (Theobroma cacao L.) leaves treated with inducers of the defense response

Joseph A. Verica, Siela Maximova, Mary D. Strem, John Edward Carlson, Bryan A. Bailey, Mark Guiltinan

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Pathogenic diseases represent a major constraint to the growth and yield of cacao (Theobroma cacao L.). Ongoing research on model plant systems has revealed that defense responses are activated via signaling pathways mediated by endogenous signaling molecules such as salicylic acid, jasmonic acid and ethylene. Activation of plant defenses is associated with changes in the expression of large numbers of genes. To gain a better understanding of defense responses in cacao, we have employed suppressive subtractive hybridization (SSH) cDNA libraries, macroarray hybridization analysis, high throughput DNA sequencing and bioinformatics to identify cacao genes induced by these signaling molecules. Additionally, we investigated gene activation by a phytotoxic elicitor-like protein, Nep1. We have identified a unigene set of 1,256 members, including 330 members representing genes induced during the defense response.

Original languageEnglish (US)
Pages (from-to)404-413
Number of pages10
JournalPlant Cell Reports
Volume23
Issue number6
DOIs
StatePublished - Nov 1 2004

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Theobroma cacao
leaves
unigenes
suppression subtractive hybridization
gene activation
genes
jasmonic acid
bioinformatics
salicylic acid
cDNA libraries
ethylene
hybridization
proteins

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

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abstract = "Pathogenic diseases represent a major constraint to the growth and yield of cacao (Theobroma cacao L.). Ongoing research on model plant systems has revealed that defense responses are activated via signaling pathways mediated by endogenous signaling molecules such as salicylic acid, jasmonic acid and ethylene. Activation of plant defenses is associated with changes in the expression of large numbers of genes. To gain a better understanding of defense responses in cacao, we have employed suppressive subtractive hybridization (SSH) cDNA libraries, macroarray hybridization analysis, high throughput DNA sequencing and bioinformatics to identify cacao genes induced by these signaling molecules. Additionally, we investigated gene activation by a phytotoxic elicitor-like protein, Nep1. We have identified a unigene set of 1,256 members, including 330 members representing genes induced during the defense response.",
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Isolation of ESTs from cacao (Theobroma cacao L.) leaves treated with inducers of the defense response. / Verica, Joseph A.; Maximova, Siela; Strem, Mary D.; Carlson, John Edward; Bailey, Bryan A.; Guiltinan, Mark.

In: Plant Cell Reports, Vol. 23, No. 6, 01.11.2004, p. 404-413.

Research output: Contribution to journalArticle

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