Isolation of JC virus capsomer-like structures from progressive multifocal leukoencephalopathy brain

Richard B. Tenser, Kenneth W. Sommerville, Jon G. Mummaw, Richard J. Frisque

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Brain tissue from a patient with progressive multifocal leukoencephalopathy (PML) was analyzed by molecular biological and electron-microscopic techniques. Viral DNA was isolated directly from brain tissue, cloned into a plasmid vector, and subjected to restriction endonuclease analysis. The pattern of restriction fragments identified by gel electrophoresis was almost indistinguishable from that of prototype JC virus. By this procedure the etiologic agent of PML in this patient was identified without the isolation of infectious virus. After centrifugal clarification of brain homogenates, high speed centrifugal pellets were studied by electron microscopy. Large numbers of 9-nm polygonal particles, sometimes in paracrystalline arrays, were observed. It was thought likely that these particles were capsomer subunits of 41-43 nm JC virus virions. That the particles were capsomers was supported by negative stain electron microscopy, including reconstruction studies with simian virus 40.

Original languageEnglish (US)
Pages (from-to)243-254
Number of pages12
JournalJournal of the neurological sciences
Volume72
Issue number2-3
DOIs
StatePublished - Feb 1986

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JC Virus
Progressive Multifocal Leukoencephalopathy
Electron Microscopy
Brain
Simian virus 40
DNA Restriction Enzymes
Viral DNA
Virion
Electrophoresis
Plasmids
Coloring Agents
Gels
Electrons
Viruses

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Tenser, Richard B. ; Sommerville, Kenneth W. ; Mummaw, Jon G. ; Frisque, Richard J. / Isolation of JC virus capsomer-like structures from progressive multifocal leukoencephalopathy brain. In: Journal of the neurological sciences. 1986 ; Vol. 72, No. 2-3. pp. 243-254.
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Isolation of JC virus capsomer-like structures from progressive multifocal leukoencephalopathy brain. / Tenser, Richard B.; Sommerville, Kenneth W.; Mummaw, Jon G.; Frisque, Richard J.

In: Journal of the neurological sciences, Vol. 72, No. 2-3, 02.1986, p. 243-254.

Research output: Contribution to journalArticle

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