Isolation of microsatellite loci in spotted salamanders (Ambystoma maculatum)

A. M. Wieczorek, K. R. Zamudio, Timothy Lee King, B. Gjetvaj

Research output: Contribution to journalReview article

6 Citations (Scopus)

Abstract

To assess the level and spatial pattern of genetic diversity in the spotted salamander, Ambystoma maculatum, we characterized hypervariable molecular markers by screening genomic libraries enriched for microsatellite motifs. We designed primers that reliably amplify twelve polymorphic loci and checked for variability in individuals from populations in the vicinity of Ithaca, New York. Loci show high variability in the number of alleles and heterozygosities, suggesting they will be useful for determining local population differentiation and mating systems in this pond-breeding amphibian.

Original languageEnglish (US)
Pages (from-to)313-315
Number of pages3
JournalMolecular Ecology Notes
Volume2
Issue number3
DOIs
StatePublished - Sep 1 2002

Fingerprint

Ambystoma
Urodela
Genomic Library
Ponds
salamanders and newts
reproductive strategy
heterozygosity
Microsatellite Repeats
amphibian
genomics
allele
Screening
pond
breeding
microsatellite repeats
loci
genomic libraries
Amphibians
mating systems
Population

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wieczorek, A. M. ; Zamudio, K. R. ; King, Timothy Lee ; Gjetvaj, B. / Isolation of microsatellite loci in spotted salamanders (Ambystoma maculatum). In: Molecular Ecology Notes. 2002 ; Vol. 2, No. 3. pp. 313-315.
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Isolation of microsatellite loci in spotted salamanders (Ambystoma maculatum). / Wieczorek, A. M.; Zamudio, K. R.; King, Timothy Lee; Gjetvaj, B.

In: Molecular Ecology Notes, Vol. 2, No. 3, 01.09.2002, p. 313-315.

Research output: Contribution to journalReview article

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AU - Gjetvaj, B.

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