Isolation of purified and live Foxp3+ regulatory T cells using facs sorting on scatter plot

Xiaohui Zhou, Julie Wang, Wei Shi, David D. Brand, Zhongmin Liu, Huimin Fan, Song Guo Zheng

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


There are no ideal ways to identify and isolate viable and purified Foxp3+ regulatory T cells so far. Here we developed a novel procedure for the isolation of highly purified Foxp3+ cells using flow cytometry. This method relies on an identification and sorting of the lymphoblast cell population identified on a scatter plot using flow cytometry. We confirmed that greater than 98% of the cells sorted using this technique expressed Foxp3 and displayed a potent suppressive activity. This method provides a valuable tool for the study of the T regulatory cell biology and their therapeutic manipulation.

Original languageEnglish (US)
Pages (from-to)164-169
Number of pages6
JournalJournal of Molecular Cell Biology
Issue number3
StatePublished - 2010

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cell Biology

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